白茶寡肽的抗氧化活性研究  

Antioxidative Activity of Oligopeptides in White Tea

作　　者：王小娟 刘慧 王良瑄 吴文晞 赵峰 WANG Xiao-juan;LIU Hui;WANG Liang-xuan;WU Wen-xi;ZHAO Feng(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China;Fujian Hengzheng Testing Technology Co.,Ltd.,Fuzhou,Fujian 350199,China;Fujian Provincial Key Laboratory for Research and Development of Traditional Chinese Medicine Resources,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122 [2]福建恒正检测技术有限公司,福建福州350199 [3]福建省中药资源重点实验室,福建福州350122

出　　处：《茶叶学报》2022年第4期233-242,共10页Acta Tea Sinica

基　　金：福建省自然科学基金项目(2020J01730);福建中医药大学引进人才科研启动项目(2801/701190097)。

摘　　要：对白茶寡肽的氨基酸序列及其抗氧化能力进行研究,将有助于了解白茶功效的物质基础。本研究首先制备白茶寡肽,对其氨基酸序列构成进行分析,然后通过测定其对1,1-二苯基-2-苦基肼(DPPH)自由基清除率、羟自由基清除率、总还原力和HepG_(2)细胞的超氧化物歧化酶(SOD)活性,进而评价其抗氧化能力。结果表明,白茶寡肽DPPH自由基清除能力的半抑制浓度(IC_(50))为0.170 mg·mL^(-1),羟自由基清除能力的IC_(50)为0.850 mg·mL^(-1);白茶寡肽的总还原力呈现浓度依赖性;进一步的细胞实验还发现白茶寡肽能使HepG_(2)氧化损伤细胞中SOD活力增强。白茶寡肽不但具有较强的体外抗氧化活性,而且对HepG_(2)细胞氧化应激损伤具有保护作用。The antioxidant capacity of the oligopeptide-rich white tea was studied by sequencing the amino acids and analyzing the related properties of the extracted oligopeptides.The rate,total reducing power,and effect on the superoxide dismutase activity of HepG_(2) cells of the oligopeptides were determined in vitro.The results indicated that the half-inhibitory concentration(IC_(50))on scavenging DPPH free radicals was 0.170 mg·mL^(-1) and that on hydroxyl radicals 0.850 mg·mL^(-1).The total reducing power of the oligopeptides was concentration dependent.The in vitro cell experiment on oxidatively damaged HepG_(2) cells showed a heightened SOD activity by the oligopeptides indicating a capacity of the functional ingredient in protectin g human cells from injury by oxidative stress.

关 键 词：白茶寡肽 抗氧化 HepG_(2)细胞 氧化应激 

分 类 号：TS272.5[农业科学—茶叶生产加工]