异鼠李素减轻糖尿病视网膜病变大鼠视网膜细胞凋亡  

Isoramin attenuates retinal cell apoptosis in diabetes retinopathy rats

作　　者：王卉 苏宪 张赢赢 褚战亚 孙朝晖 张金玲 张倩倩 WANG Hui;SU Xian;ZHANG Yingying;CHU Zhanya;SUN Zhaohui;ZHANG Jinling;ZHANG Qianqian(Department of Ophthalmology,Shijiazhuang People's Hospital,Shijiazhuang 050030,China;Outpatient Department,Shijiazhuang People's Hospital,Shijiazhuang 050030,China)

机构地区：[1]石家庄市人民医院眼科,河北石家庄050030 [2]石家庄市人民医院门诊部,河北石家庄050030

出　　处：《基础医学与临床》2023年第2期265-270,共6页Basic and Clinical Medicine

基　　金：2021年度河北省医学科学研究课题计划(20210807)。

摘　　要：目的探讨异鼠李素对糖尿病视网膜病变(DR)模型大鼠视网膜细胞凋亡的影响。方法将大鼠分为对照组(control)、DR模型组(model)、异鼠李素低、高剂量组(ISO-L、ISO-H,分别口服50、150 mg/kg异鼠李素)、阳性药物组(positive drug,口服150 mg/kg羟苯磺酸钙),ELISA法检测血清炎性因子水平;HE染色及TUNEL染色观察视网膜病理变化及细胞凋亡,Western blot法检测视网膜组织VEGF、Bax、caspase-3蛋白表达和p38 MAPK、NF-κB p65磷酸化水平。结果与对照组比较,model组大鼠视网膜排列紊乱,分界不清,内、外核层水肿,细胞数量减少,视网膜厚度变薄,视网膜细胞凋亡率、血清TNF-α、IL-1β、IL-6水平、VEGF、Bax、caspase-3蛋白表达及p38 MAPK、NF-κB p65磷酸化水平显著升高(P<0.05);与model组比较,ISO-L组、ISO-H组、阳性药物组大鼠视网膜组织各层界限较清晰,结构稍显疏松,视网膜厚度增加,视网膜细胞凋亡率、血清TNF-α、IL-1β、IL-6水平、VEGF、Bax、caspase-3蛋白表达及p38 MAPK、NF-κB p65磷酸化水平显著降低(P<0.05)。结论异鼠李素可能通过抑制MAPK/NF-κB信号通路,减轻DR大鼠视网膜损伤及细胞凋亡。Objective To investigate the effect of isorhamnetin on the apoptosis of retinal cells in diabetic retinopathy(DR)rats.Methods The rats were divided into control group,DR model group,low and high dose isorhamnetin groups(ISO-L group,ISO-H group,50,150 mg/kg isorhamnetin orally,respectively)and positive drug group(150 mg/kg calcium dobesilate orally).The level of serum inflammatory factors was measured by ELISA;HE staining and TUNEL staining were performed to observe the pathological changes and cell apoptosis of retina.Western blot method was performed to detect protein expression of VEGF,Bax,caspase-3 and phosphorylation levels of p38 MAPK,NF-κB p65 in retinal tissue.Results Compared with the control group,the retina of the model group was arranged disorderly,the boundary was unclear,the inner and outer nuclear layers were edema,the number of cells was reduced,and the thickness of the retina became thinner,and the retinal cell apoptosis rate,serum TNF-α,IL-1β,IL-6 levels,VEGF,Bax,caspase-3 protein expression and p38 MAPK,NF-κB p65 phosphorylation were significantly increased(P<0.05);Compared with the model group,the retinal tissue layers of the ISO-L,ISO-H,and positive drug groups had clearer boundaries,slightly looser structures,and increased retinal thickness,and the retinal cell apoptosis rate,serum TNF-α,IL-1β,IL-6 levels,VEGF,Bax,caspase-3 protein expression,and p38 MAPK,NF-κB p65 phosphorylation levels were significantly reduced(P<0.05).Conclusions Isorhamnetin may ameliorate retinal injury and cell apoptosis in DR rats by inhibiting the MAPK/NF-κB signaling pathway.

关 键 词：异鼠李素 糖尿病视网膜病变 视网膜组织 MAPK/NF-κB信号通路 细胞凋亡 

分 类 号：R774.1[医药卫生—眼科]