河西走廊产区本土酒酒球菌高密度培养工艺条件优化  

Optimization of High Density Culture Conditions of Native Oenococcus oeni in Hexi Corridor

作　　者：于静 毛亚玲 李俊娥 杨柳 祝霞[1,2] 杨学山 YU Jing;MAO Yaling;LI June;YANG Liu;ZHU Xia;YANG Xueshan(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou Gansu 730000,China;Gansu Key Laboratory of Viticulture and Enology,Lanzhou Gansu 730000,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730000 [2]甘肃省葡萄与葡萄酒工程学重点实验室,甘肃兰州730070

出　　处：《食品与发酵科技》2022年第6期23-31,共9页Food and Fermentation Science & Technology

基　　金：国家自然科学基金项目(32060581);甘肃省自然科学基金项目(20JR10RA527);甘肃省葡萄酒产业发展基金项目(20180820-07,20180820-08);甘肃农业大学校列项目(GSAU-ZL-2018-8)。

摘　　要：为优化本土酒酒球菌(Oenococcus oeni,O.oeni)高密度培养工艺条件,以分离自甘肃河西走廊产区的本土O.oeni ZX-1和WW-1为试验菌株,利用单因素试验结合Box-Behnken设计对菌株的培养条件进行优化。结果表明,酒酒球菌ZX-1的最佳培养条件:培养温度28℃,初始p H 5.06,碳源含量15.1 g/L;酒酒球菌WW-1的最佳培养条件:培养温度28℃,初始p H 5.08,碳源含量15.3 g/L。在最佳培养条件下,2株本土菌株发酵液活菌数均达到1.0×10^(9)CFU/mL,与模型预测值无显著差异(P>0.05)。综上,优化后的高密度培养工艺可有效提高本土O.oeni菌株ZX-1和WW-1的活菌数,对于制备高质量本土O.oeni直投式发酵剂具有重要意义。In order to optimize the high density culture conditions of native Oenococcus oeni.ZX-1 and WW-1isolated from Hexi corridor of Gansu province were used as test strains.And the culture conditions of the strain were optimized by single factor test combined with Box-Behnken design.The results showed that the optimal culture conditions of ZX-1 were:culture temperature 28℃,initial pH 5.06,carbon source content 15.1 g/L;the optimal culture conditions of WW-1 were:culture temperature 28℃,initial pH 5.08,carbon source content15.3 g/L.Under the optimal culture conditions,the viable count in the fermentation liquid of the two native strains reached 1.0×10^(9)CFU/mL,which was not significantly different from the predicted value of the model(P>0.05).In conclusion,the optimized high density culture process can effectively increase the number of viable counts of native O.oeni strains ZX-1and WW-1,which is of great significance for the preparation of high-quality native O.oeni direct vat set(DVS).

关 键 词：高密度培养 活菌数 本土酒酒球菌 BOX-BEHNKEN设计 

分 类 号：TS261.13[轻工技术与工程—发酵工程]