基于全基因组信息的常山胡柚遗传鉴定  

Genetic identification of Changshan Huyou based on whole genome information

作　　者：毛桑隐 汪丽霞 宋剑锋 刘胜军 王沦 徐强[1] MAO Sangyin;WANG Lixia;SONG Jianfeng;LIU Shengjun;WANG Lun;XU Qiang(College of Horticulture and Forestry Sciences,Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology,Ministry of Education,Wuhan 430070,Hubei,China;Changshan Characteristic Industry Development Center,Changshan 324000,Zhejiang,China;Quzhou Institute for Food and Drug Control,Quzhou 324000,Zhejiang,China)

机构地区：[1]华中农业大学园艺林学学院·园艺植物生物学教育部重点实验室,武汉430070 [2]常山县特色产业发展中心,浙江常山324000 [3]衢州市食品药品检验研究院,浙江衢州324000

出　　处：《果树学报》2023年第1期25-34,共10页Journal of Fruit Science

基　　金：国家重点研发计划项目(2018YFD1000101);中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302);浙江省农业新品种选育重大科技专项(2021C02074);国家自然科学基金杰青项目(31925034)。

摘　　要：【目的】常山胡柚是我国一个优良地方柑橘资源,但对其遗传背景一直存在争议,有必要进一步探究常山胡柚的遗传背景。【方法】本研究基于全基因组重测序数据结果,并用分子标记进行验证,同时结合形态特征以及果实品质数据,将常山胡柚与相关柑橘资源进行系统比较。【结果】基因组重测序数据表明,常山胡柚中超过87.91%的遗传组分为柚橘杂合,柚纯合的遗传组分为9.77%,且全基因组序列与酸橙相似度较高,其中与江津酸橙序列相似度达88.34%,表明常山胡柚是一种酸橙。根据不同位点开发的4对InDel分子标记进行检测,也印证了常山胡柚与酸橙亲缘关系较近的结论。表型和品质数据表明,常山胡柚在叶片形态上与酸橙相近,且翼叶较小,果实形态类似酸橙,且单果质量、果皮厚度、种子质量、果皮质量等形态指标也与酸橙无显著性差异,而与橘、柚及葡萄柚存在显著差异。常山胡柚可滴定酸含量、固酸比等品质指标较酸橙明显优化。【结论】常山胡柚为酸橙遗传背景,是经长期栽培驯化后形成的一种果实品质改良的酸橙。【Objective】Changshan Huyou is an excellent local citrus resource in China,but its genetic background is always controversial.The study aimed to explore the genetic background of Changshan Huyou.【Methods】Changshan Huyou was used as the experimental material.Other tested materials included typical citrus species resources and local citrus resources in Zhejiang,such as mandarin orange,pummelo and sour orange.Firstly,the morphological characteristics of leaves and fruits of Changshan Huyou were compared with mandarin orange,pummelo,sour orange and grapefruit.Secondly,the fruit quality of different citrus resources was determined,the transverse and longitudinal diameter of fruit was measured by vernier caliper,and the fruit shape index was calculated,the weights of single fruit,peel,residue and seed were measured by electronic balance,and the edible rate was calculated,the soluble solids and titratable acids were measured by hand-held sugar acid instrument,and the solid acid ratio of fruit was calculated,the content of ascorbic acid was determined by 2,6-dichlorophenol indophenol reagent.The differences in appearance quality and internal quality of the various resources were analyzed.The genome of Changshan Huyou was resequenced,and the sweet orange was used as the reference genome.Changshan Huyou was compared with the sweet orange reference genome through gatk4.0 for SNP-calling,and finally the SNP data of Changshan Huyou,mandarin,sour orange,pummelo and grapefruit were obtained.The SNP information in each window was analyzed,so as to judge the genetic source of each window and obtain the genetic component map of their whole genome.Based on the genomic difference information of mandarin orange,pummelo,Changshan Huyou,sour orange and grapefruit,specific InDel molecular markers were developed,and Changshan Huyou was further identified by the molecular markers.【Results】The morphological analysis of leaves and fruits showed that there were great differences in leaf types of different citrus resources.The leaf

关 键 词：常山胡柚 酸橙 基因组分析 分子标记 果实品质 

分 类 号：S666.3[农业科学—果树学]