ERBIN调控PDZD2蛋白表达对结直肠癌细胞增殖、侵袭及凋亡的影响  

Effect of PDZD2 protein regulated by ERBIN expression on proliferation,invasion and apoptosis of colorectal cancer cells

作　　者：盛仁明 蔡为民 段鸿梅 孙丽娜 涂健[2] Sheng Renming;Cai Weimin;Duan Hongmei;Sun Lina;Tu Jian(Dept of Pathology,Changshu Hospital Affiliated to Soochow University,Changshu No.1 People′s Hospital,Changshu 215500;Dept of Pathology,The Second Affiliated Hospital of Soochow University,Suzhou 215004)

机构地区：[1]苏州大学附属常熟医院(常熟市第一人民医院)病理科,常熟215500 [2]苏州大学附属第二医院病理科,苏州215004

出　　处：《安徽医科大学学报》2022年第12期1933-1940,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82072690)。

摘　　要：目的探讨结直肠癌组织中表皮生长因子受体2相互作用蛋白(ERBIN)和包含盘状同源区域蛋白2(PDZD2)蛋白的表达及临床意义,以及ERBIN和PDZD2对结直肠癌进展的影响及相应机制。方法采用免疫印迹法检测来自86对结直肠癌组织样本(癌组织组)和对应距肿瘤边缘5 cm的癌旁正常组织(癌旁组织组)、正常人结肠成纤维细胞系(CCD-18Co)和人结直肠癌细胞系(SW480、HCT116、SW620和HT29)中ERBIN和PDZD2蛋白的表达;结直肠癌细胞SW620和HCT116转染ERBIN或PDZD2过表达载体后,MTT法检测细胞增殖,Transwell法检测细胞侵袭,流式细胞术检测细胞凋亡,免疫荧光化学观察ERBIN或PDZD2蛋白在SW620细胞中的表达,蛋白质免疫共沉淀实验检测ERBIN和PDZD2蛋白的相互作用。结果结直肠癌组织中ERBIN和PDZD2的表达水平低于癌旁组织(P<0.01),结直肠癌细胞中ERBIN和PDZD2的表达水平低于CCD-18Co细胞(P<0.01);ERBIN蛋白的表达水平与结直肠癌TNM分期和淋巴结转移相关联(P<0.001),PDZD2蛋白的表达水平与结直肠癌的浸润深度和分化程度显著相关(P<0.001);ERBIN或PDZD2过表达后,结直肠癌SW620和HCT116细胞增殖和侵袭能力降低(P<0.01),细胞凋亡升高(P<0.01);ERBIN和PDZD2蛋白可直接结合,ERBIN过表达可提高PDZD2蛋白的表达水平。结论ERBIN通过调控PDZD2蛋白的表达,抑制结直肠癌细胞增殖、侵袭,促进凋亡。Objective To investigate the expression and clinical significance of erbb2 interacting protein(ERBIN)and PDZ domain containing 2 protein(PDZD2)in colorectal cancer tissues,as well as the effects of ERBIN and PDZD2 on the progression of colorectal cancer and the corresponding mechanisms.Methods Western blot was used to detect the expression of ERBIN and PDZD2 proteins in tumor tissues and corresponding adjacent tissues from 86 colorectal cancer patients,as well as normal human colon fibroblasts(CCD-18Co)and human colorectal cancer cell lines(SW480,HCT116,SW620 and HT29);SW620 and HCT116 cells were transfected with ERBIN or PDZD2 overexpression vector,and then cell proliferation,cell invasion and apoptosis were detected.The expression of ERBIN or PDZD2 protein in SW620 cell was observed by immunofluorescence chemistry.Co-immunoprecipitation assay was used to detect the interaction between ERBIN and PDZD2 proteins.Results The expression levels of ERBIN and PDZD2 proteins in colorectal cancer tissues were lower than those in adjacent tissues(P<0.01),and the expression levels of ERBIN and PDZD2 in colorectal cancer cells were lower than those in CCD-18Co cells(P<0.01).The expression levels of ERBIN and PDZD2 proteins were correlated with the depth of invasion,differentiation,TNM staging and lymph node metastasis of colorectal cancer(P<0.001);ERBIN or PDZD2 overexpression reduced the proliferation and invasion of SW620 and HCT116 cells(P<0.01),increased cell apoptosis(P<0.01);ERBIN directly bound to PDZD2,and overexpression of ERBIN increased the expression level of PDZD2 protein.Conclusion ERBIN can inhibit the proliferation and invasion of colorectal cancer cells and promote apoptosis by promoting the expression of PDZD2 protein.

关 键 词：结直肠癌 ERBIN PDZD2 临床病理学 细胞增殖和侵袭 

分 类 号：R735.3[医药卫生—肿瘤]