过表达KLF7对食管鳞状癌细胞的增殖和迁移的影响  

Effect of overexpression of KLF7 on proliferation and migration of esophageal squamous cell carcinoma

作　　者：陈晶晶 张志威[1] 慕晓玲[1] Chen Jingjing;Zhang Zhiwei;Mu Xiaoling(Dept of Histology and Embryology,Medical College,Shihezi University,Shihezi 832000)

机构地区：[1]石河子大学医学院组织胚胎学教研室,石河子832000

出　　处：《安徽医科大学学报》2023年第1期120-126,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:31460310);石河子大学拔尖人才项目(编号:CXBJ201905)。

摘　　要：目的探索Kruppel-like factor 7(KLF7)对食管鳞癌细胞Eca109细胞增殖、迁移和周期的影响。方法利用生物信息学方法研究KLF7在食管鳞癌组织中的表达模式及其与患者预后、病理分级的关系。利用Real-time PCR、Western blot与细胞免疫荧光技术研究食管鳞癌细胞中KLF7的表达模式与亚细胞定位。利用KLF7过表达质粒(pcDNA-3.10-KLF7)细胞转染实现KLF7在Eca109食管鳞癌细胞中过表达。采用平板克隆计数和CCK-8检测研究细胞增殖能力,利用Transwell研究细胞迁移能力,利用流式细胞术检测细胞周期。结果KLF7在食管癌组织和细胞中均有表达,其表达水平与患者病理分级显著相关(P<0.05),与患者生存率无显著相关性。食管癌细胞系(Eca109和EC9706)中KLF7的表达水平显著高于正常食管上皮细胞(Het-1A,P<0.05),并且Het-1A细胞中KLF7高表达于细胞质和细胞核,而2种食管癌细胞Eca109和EC9706中KLF7仅高表达于细胞核,在Eca109细胞中过表达KLF7后改变细胞周期、促进细胞的增殖和迁移以及CCND1和P53的表达水平上调(P<0.05)。结论KLF7的过表达可能促进食管癌细胞的增殖和迁移。Objective To explore the effects of Kruppel-like factor 7(KLF7)on the proliferation,migration and cycle of esophageal squamous cell carcinoma cell line Eca109.Methods Bioinformatics analysis was used to study the expression pattern of KLF7 in esophageal squamous cell carcinoma and its relationship with prognosis and pathological grade.The expression pattern of KLF7 in esophageal squamous cell carcinoma cells was studied by real-time PCR,Western blot and cellular immunofluorescence in vitro.The KLF7 overexpression in Eca109 cells was carried out by transfection of the plasmid of pcDNA-3.10-KLF7,and the effects of KLF7 on the proliferation and migration of ESCC cells were detected by CCK-8 assay,plate clone formation assay and Transwell assay,and the cell cycle was studied by Flow cytometric analysis.Results KLF7 was expressed in the tissues and cells of esophageal cancer,and its expression was significantly associated with the pathological grade of esophageal cancer patients(P<0.05),however,there was no significant associaition of KLF7 expression with the survival rate of patients.The expression level of KLF7 in esophageal cancer cells(Eca109 and EC9706)was significantly higher than that in normal esophageal epithelial cells(Het-1A,P<0.05).In addition,the expression of KLF7 was higher in the cytoplasm and nucleus of Het-1A cells,while only in the nucleus of esophageal cancer cells(Eca109 and EC9706).Additionally,the overexpression of KLF7 in Eca109 cells changed the cell cycle,promoted cell proliferation and migration,and up-regulated the expression levels of CCND1 and P53(P<0.05).Conclusion Overexpression of KLF7 might promote the proliferation and migration of esophageal cancer cells.

关 键 词：食管癌 Kruppel-like factor 7 增殖 迁移 P53 CCND1 

分 类 号：R73[医药卫生—肿瘤] Q279[医药卫生—临床医学] R32[生物学—细胞生物学]