雪菊茶总黄酮抑制家兔离体十二指肠收缩及机制研究  

Inhibitory effect of the flavonoids from Coreopsis tinctoria Nutt.on the rabbits duodenal contraction in vitro and its mechanism

作　　者：沙爱龙 郝海燕 SHA Ailong;HAO Haiyan(School of Teacher Education,Chongqing Three Gorges University,Chongqing 404120;School of Environmental and Chemical Engineering,Chongqing Three Gorges University,Chongqing 404120)

机构地区：[1]重庆三峡学院教师教育学院,重庆404120 [2]重庆三峡学院环境与化学工程学院,重庆404120

出　　处：《中国食品添加剂》2022年第12期75-80,共6页China Food Additives

基　　金：国家自然科学基金(31360635);重庆市渝东北特色生物资源开发利用工程技术研究中心开放基金项目(19sgxyjdzz12);重庆市教育委员会科学技术研究项目(KJQN201901235;KJQN202001226);重庆三峡学院人才引进科研启动金项目(20190002)。

摘　　要：目的:研究雪菊茶总黄酮对家兔离体十二指肠收缩的抑制作用及机制。方法:采用Medlab生物信号系统和分光光度计检测雪菊茶总黄酮对离体兔肠收缩抑制及机制。结果:雪菊茶总黄酮能抑制兔离体肠收缩。20 mg/mL的雪菊茶总黄酮能协同(P<0.05)AD抑制肠收缩,能显著(P<0.05)抑制CaCl_(2)对其促进作用。紫外-可见分光光度检测显示,雪菊茶总黄酮3个浓度组家兔离体肠中ChAT活性均低于正常对照组,A-CHE和TNOS活性均升高。结论:黄酮是雪菊茶中抑制十二指肠收缩的有效成分,作用机制与激动β受体、促进NO合成从而激活GC-cGMP-PKG信号通路、抑制ACh从而抑制AC-cAMP-PKA信号通路、抑制肌膜I_(Ca-L)从而抑制胞外Ca^(2+)内流等有关。Objective:To investigate the effects of the flavonoids of Coreopsis tinctoria Nutt.(CTFS)on the contractile activity of the rabbit duodenum in vitro,and to explore the mechanism.Methods:The Medlab biosignal system and spectrophotometer were used to detect the inhibition and mechanism of CTFS on contractile activity of the rabbit duodenum in vitro.Results:The CTFS could inhibit the contraction of duodenum.The CTFS could synergize(P<0.05)the inhibition effect of AD on the intestinal contraction,and significantly(P<0.05)inhibit the promotion effect of Ca Cl_(2).The UV-vis detection showed that the Ch AT activity of the three concentration groups of CTFS was decreased,the activities of A-CHE and TNOS were increased,compared with the normal control group,.Conclusion:The fiavonoids were effective component of Coreopsis tinctoria in inhibiting the duodenum movement.The mechanism may be related to the inhibitions of Ach,AC-c AMP-PKA signal pathway and I_(Ca-L) in sarcolemma,the promotion of NO synthesis and the activations of GC-c GMP-PKG signaling pathway andβreceptor.

关 键 词：雪菊茶 黄酮 十二指肠平滑肌 机制 CA^(2+) 神经递质 信号通路 

分 类 号：TS202.3[轻工技术与工程—食品科学]