中华跌打丸调节钙通道及ATP代谢改善外周疲劳作用研究  

Study on Anti-peripheral Fatigue Effect of Zhonghua Dieda Pills by Regulating Calcium Channel and ATP Metabolism

作　　者：张明哲 戴明珠 梁云飞 郝二伟 侯小涛 邓家刚 叶祖光[5] 吕林艳 刘洋 杜正彩[1] ZHANG Mingzhe;DAI Mingzhu;LIANG Yunfei;HAO Erwei;HOU Xiaotao;DENG Jiagang;YE Zuguang;LYU Linyan;LIU Yang;DU Zhengcai(Guangxi University of Traditional Chinese Medicine,Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Guangxi Scientific Experimental Center of Traditional Chinese Medicine,Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica,Nanning 530200,China;Hunter Biotechnology Group Co.,Ltd.,Hangzhou 310051,China;Guangxi Wuzhou Pharmaceutical Group Co.,Ltd.,Wuzhou 543000,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100000,China)

机构地区：[1]广西中医药大学广西中医药科学实验中心,南宁530200 [2]广西中医药大学广西中药药效研究重点实验室,南宁530200 [3]杭州环特生物科技股份有限公司,杭州310051 [4]广西梧州制药(集团)股份有限公司,广西梧州543000 [5]中国中医科学院中药研究所,北京100000

出　　处：《中国现代应用药学》2022年第24期3234-3239,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：广西科技重大专项(桂科AA17202040-2);广西中药药效研究重点实验室项目(17-259-20)。

摘　　要：目的 建立缺氧-疲劳斑马鱼模型,研究中华跌打丸抗外周疲劳作用及其机制。方法 利用亚硫酸钠诱导建立缺氧-疲劳模型,分别从运动能力、乳酸含量、ATP生成、镇痛、自由基清除和钙离子代谢6个方面考察中华跌打丸的抗疲劳作用。结果 中华跌打丸(250,500,1 000和2 000μg·mL^(-1))可明显改善疲劳斑马鱼的运动能力,促进乳酸代谢,降低机体乳酸含量,同时具有镇痛作用;中华跌打丸1 000μg·mL^(-1)可明显促进疲劳斑马鱼ATP能量合成;中华跌打丸500,1 000和2 000μg·mL^(-1)可明显提高超氧化物歧化酶1(SOD1)基因相对表达量;中华跌打丸1 000,2 000μg·mL^(-1)可明显提高钙离子通道(CACNAIDA)基因相对表达量。结论 中华跌打丸具有明显的抗外周疲劳和镇痛作用,其机制可能与促进乳酸代谢、促进ATP生成、提高SOD1和CACNA1DA基因相对表达量有关。OBJECTIVE To establish the zebrafish hypoxia-fatigue model,and to study the anti-peripheral fatigue effect of Zhonghua Dieda pills and its mechanism.METHODS The hypoxia-fatigue model of zebrafish was established by sodium sulfite.The anti-fatigue effects of Zhonghua Dieda pills were observed from six aspects:exercise,content of lactic acid,ATP production,degree of pain,free radical scavenging and calcium ion metabolism.RESULTS Zhonghua Dieda pills(250,500,1 000 and 2 000μg·mL^(-1)) could significantly improve the exercise ability of fatigued zebrafish,promote lactic acid metabolism,and reduce the content of lactic acid.Zhonghua Dieda pills 1 000μg·mL^(-1)could significantly promote ATP energy synthesis of fatigued zebrafish,while having an analgesic effect.Zhonghua Dieda pills 500,1 000 and 2 000μg·mL^(-1)could significantly increase the relative expression of SOD1 gene.Zhonghua Dieda pills 1 000,2 000μg·mL^(-1)could significantly increase the relative expression of CACNA1DA gene.CONCLUSION Zhonghua Dieda pills has obvious anti-peripheral fatigue and analgesic effect,the mechanism maybe related to promoting lactate metabolism,promoting ATP production,increasing the relative expression of SOD1 gene and increasing the relative expression of CACNA1DA gene.

关 键 词：斑马鱼 中华跌打丸 抗疲劳 乳酸 ATP 超氧化物歧化酶1 钙离子通道 

分 类 号：B285.5[哲学宗教—中国哲学]