Fluorescence life-time imaging microscopy(FLIM)monitors tumor cell death triggered by photothermal therapy with MoS_(2) nanosheets  

作　　者：Hongda Liang Zheng Peng Xiao Peng Yufeng Yuan Teng Ma Yiwan Song Jun Song Junle Qu 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University,Shenzhen 518060,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2019年第5期69-78,共10页创新光学健康科学杂志（英文）

基　　金：supported by the National Key R&D Program of China(2018YFC0910602);the National Natural Science Foundation of China(Grant Nos.31771584/61775145/61605121,61620106016/61525503/61835009/81727804);Guangdong Natural Science Foundation Innovation Team(2014A030312008);Shenzhen Basic Research Project(JCYJ20170818100153423/JCYJ20170412110212234/JCYJ20160328144746940/JCYJ20170412105003520/JCYJ20170302142902581);Science Foundation of SZU(Grant No.000193).

摘　　要：Recently,photothermal therapy(PTT)has been proved to have great potential in tumor therapy.In the last several years,MoS_(2),as one novel member of nanomaterials,has been applied into PTT due to its excellent photothermal conversion efficacy.In this work,we applied fuorescence lifetime imaging microscopy(FLIM)techniques into monitoring the PPT-triggered cell death under MoS_(2) nanosheet treatment.Two types of MoS_(2) nanosheets(single layer nanosheets and few layer nanosheets)were obtained,both of which exhibited presentable photothermal conversion fficacy,leading to high cell death rates of 4T1 cells(mouse breast cancer cells)under PTT.Next,live cell images of 4T1 cells were obtained via directly labeling the mitochondria with Rodamine123,which were then continuously observed with FLIM technique.FLIM data showed that the fuorescence lifetimes of mitochondria targeting dye in cells treated with each type of MoS_(2) nanosheets significantly increased during PTT treatment.By contrast,the fuorescence lifetime of the same dye in control cells(without nanomaterials)remained constant after laser irradiation.These findings suggest that FLIM can be of great value in monitoring cell death process during PTT of cancer cells,which could provide dynamic data of the cellular microenvironment at single cell level in multiple biomedical applications.

关 键 词：Fluorescence lifetime imaging microscopy(FLIM) MoS_(2)nanosheets photothermal therapy(PTT) 4T1 cells 

分 类 号：R730.5[医药卫生—肿瘤]