Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications  

作　　者：Mengjie Zhao Shuai Ye Xiao Peng Jun Song Junle Qu 

机构地区：[1]Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic Engineering Shenzhen University,Shenzhen 518060,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2019年第5期79-85,共7页创新光学健康科学杂志（英文）

基　　金：supported by the National Key R&D Program of China(2018YFC0910600);the National Natural Science Foundation of China(61605124/41603059/61525503/61620106016/61835009/81727804);Project of Department of Education of Guangdong Province(2015KGJHZ002/2016KCXTD007);Guangdong Natural Science Foundation Innovation Team(2014A030312008);Shenzhen Basic Research Project(JCYJ20150930104948169/JCYJ20160328144746940/JCYJ20170412105003520);the Natural Science Foundation of Shenzhen University(2019108).

摘　　要：Inorganic quantum dots(QDs)have excellent optical properties,such as high°uorescence intensity,excellent photostability and tunable emission wavelength,etc.,facilitating them to be used as labels and probes for bioimaging.In this study,CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope(FLIM)and stimulated emission depletion(STED)nanoscopy imaging.The emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield(PLQY)was 64%.The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8 mW.The lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser(27.5 mW)irradiation.Furthermore,the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5 nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes.Meanwhile,Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM,and their fluorescence lifetime was around 3.1 ns,consistent with the in vitro value,suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.

关 键 词：CdSe@ZnS QDs FLIM STED living cells 

分 类 号：O413[理学—理论物理]