Monitoring of time-resolved singlet oxygen luminescence at 1270 nm by an optical fiber detection system  

作　　者：Cheng Zhang Hongxin Lin Ying Hu Jian Sui Lisheng Lin Buhong Li 

机构地区：[1]Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education Fujian Provincial Key Laboratory of Photonics Technology Fujian Normal University Fuzhou,Fujian 350007,P.R.China [2]Department of Gastrointestinal Surgery Fujian Provincial Hospital Fuzhou,Fujian 350013,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2022年第6期68-77,共10页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(61935004);the Natural Science Foundation of Fujian Province(2020J01155,2022J01171).

摘　　要：Singlet oxygen(^(1)O_(2))is the main cytotoxic substance in Type II photodynamic therapy(PDT).The luminescence of ^(1)O_(2) at 1270nm is extremely weak with a low quantum yield,making the direct detection of'O2 at 1270 nm very challenging.In this study,a set of highly sensitive optical fiber detection system is built up to detect the luminescence of photosensitized ^(1)O_(2) We use this system to test the luminescence characteristics of ^(1)O_(2) in pig skin tissue ex vrivo and mouse auricle skin in vivo.The experimental results show that the designed system can quantitatively detect photosensitized ^(1)O_(2) luminescence.The ^(1)O_(2) luminescence signal at 1270 nm is successfully detected in pig skin exr vivo.Compared with RB in an aqueous solution,the lifetime of ^(1)O_(2) increases to 17.4±12pus in pig skin tissue ex vivo.Experiments on living mice suggest that an enhancement of ^(1)O_(2) intensity with the increase of the TMPyP concentration.When the dose is 25 mg/kg,the vasoconstriction can reach more than 80%。The results of this study hold the potential application for clinical PDT dose monitoring using an optical fiber detection system.

关 键 词：Singlet oxygen photodynamic therapy optical fiber detection system VASOCONSTRICTION 

分 类 号：R730.5[医药卫生—肿瘤]