Engineering of Reversible Luminescent Probes for Real-Time Intravital Imaging of Liver Injury and Repair  

作　　者：Xiao Liu Linhui He Xiangyang Gong Yue Yang Dan Cheng Juanjuan Peng Lu Wang Xiao-Bing Zhang Lin Yuan 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082 [2]State Key Laboratory of Natural Medicines,School of Basic Medical Sciences and Clinical Pharmacy,China Pharmaceutical University,Nanjing,Jiangsu 211198 [3]Department of Chemical Biology,Max Planck Institute for Medical Research,Heidelberg 69120

出　　处：《CCS Chemistry》2022年第1期356-368,共13页中国化学会会刊（英文）

基　　金：supported by NSFC(nos.21877029,22074036,21735001);the National Key R&D Program of China(no.2019YFA0210100).

摘　　要：As the major organ for drug metabolism and detoxification,the liver is prone to damage and severely impaired functionality.The treatment of liver diseases is based on a clear understanding of the process underlying liver injury and repair.However,intravital real-time imaging of liver injury and repair is still limited due to the lack of in vivo reversible visualization methods.To this end,we proposed a rational design strategy for the development of a reversible upconversion luminescence nanoprobe that allows real-time and in vivo imaging of liver injury and repair processes.As a proof of concept,we first developed a small molecule probe NB3 which can reversibly respond to related analytes of early liver injury[peroxynitrite(ONOO−)]and liver repair[glutathione(GSH)].The small molecule probe was then integrated with a core–shell upconversion nanoparticle to form a sophisticated nanoprobe.Compared with traditional small molecule probes,this nanoprobe exhibited a higher selectivity to ONOO−,longer retention time in liver,and wider dynamic response range to GSH after oxidation by ONOO−.The novel nanoprobe facilitated the successful monitoring and discrimination among the different degrees of liver injury and repair in a mouse model.

关 键 词：luminescent probes liver injury and repair fluorescence imaging PEROXYNITRITE GSH 

分 类 号：O657.3[理学—分析化学] R575[理学—化学]