Wireless Electrochemical Visualization of Intracellular Antigens in Single Cells  

作　　者：Yuling Wang Dechen Jiang Hong-Yuan Chen 

机构地区：[1]State Key Laboratory of Analytical Chemistry for Life Science,Chemistry and Biomedicine Innovation Center(ChemBIC),School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,Jiangsu 210093

出　　处：《CCS Chemistry》2022年第7期2221-2227,共7页中国化学会会刊（英文）

基　　金：supported by the Ministry of Science and Technology of China(no.2017YFA0700500);the National Natural Science Foundation of China(nos.21874069,21974060,and 22025403).

摘　　要：Electrochemical microscopy has been developed in the past decades for imaging biomolecules in single cells;however,electrochemical visualization of an intracellular protein in one cell is difficult.Herein,for the first time,we have developed a model whereby the protein,KDM1/LSD1 antigen,in the nucleus of a single MCF 7 cell could be visualized using wireless bipolar electrochemiluminescence(BPEECL).Submicron-sized,single-walled carbon nanotubes were linked with anti-KDM1/LSD1 antibodies and loaded into the cells for the recognition of the corresponding KDM1/LSD1 antigen.With a low electric field of 1000 V/cm,L012,a luminol analog,was oxidized electrochemically at one end of the nanotube that emitted light for the wireless visualization of its location.The significant drop in the applied voltage permitted the observation of apparent ECL emission inside the nucleus of a single cell,supporting the electrochemical imaging of intracellular KDM1/LSD1 antigen at a single-cell level.This work solves a long-standing task in the field of electroanalysis for intracellular wireless visualization,which should advance the development of electrochemistry in single-cell analysis.

关 键 词：bipolar electrochemiluminescence electrochemical visualization intracellular proteins SINGLE-CELL carbon nanotubes 

分 类 号：O646[理学—物理化学]