The RNA N^(6)-methyladenosine demethylase ALKBH9B modulates ABA responses in Arabidopsis  

作　　者：Jun Tang Junbo Yang Qiang Lu Qian Tang Shuyan Chen Guifang Jia 

机构地区：[1]Synthetic and Functional Biomolecules Center,Bejing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,Department of Chemical Biology,College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China [2]Peking-Tsinghua Center for Life Sciences,Beijing 100871,China

出　　处：《Journal of Integrative Plant Biology》2022年第12期2361-2373,共13页植物学报（英文版）

基　　金：the Postdoctoral Fellowship of Peking-Tsinghua Center for Life Sciences;supported by the National Natural Science Foundation of China(nos.22225704,21820102008,92053109);the National Basic Research Program of China(2019YFA0802201);the Beijing Natural Science Foundation(Z200010)。

摘　　要：The mRNA modification N^(6)-methyladenosine(m^(6)A)plays vital roles in plant development and biotic and abiotic stress responses.The RNA m^(6)A demethylase ALKBH9 B can remove m^(6)A in alfalfa mosaic virus RNA and plays roles in alfalfa mosaic virus infection in Arabidopsis.However,it is unknown whether ALKBH9 B also exhibits demethylation activity and has a biological role in endogenous plant mRNA.We demonstrated here that mRNA m^(6)A modification is induced by the phytohormone abscisic acid(ABA)and that ALKBH9 B has m^(6)A demethylation activity on endogenous mRNA.Knocking out ALKBH9 B led to hypersensitivity to ABA treatment during seed germination and early seedling development.We further showed that ALKBH9 B removes the m^(6)A modification in the ABA INSENSITIVE 1(ABI1)and BRI1-EMS-SUPPRESSOR 1(BES1)transcripts following ABA treatment,affecting the stability of these mRNAs.Furthermore,we determined that ALKBH9 B acts genetically upstream of the transcription factors ABI3 and ABI5,and its regulatory function in ABA responses depended on ABI3 and ABI5.Our findings reveal the important roles of the m^(6)A modification in ABA responses and highlight the role of ALKBH9 Bmediated m^(6)A demethylation in regulating ABA responses post-transcriptionally.

关 键 词：ABA ALKBH9B ARABIDOPSIS DEMETHYLASE m^(6)A RNA modification 

分 类 号：Q943[生物学—植物学]