紫草素对血管瘤内皮细胞增殖、凋亡、迁移及成血管的影响  

Effect of shikonin on proliferation,apoptosis,migration and angiogenesis of hemangioma endothelial cell

作　　者：杨恩黎 王娜 李名扬 李易铭 耿一鸣 张东升 吴海威 YANG En-li;WANG Na;LI Ming-yang;LI Yi-ming;GENG Yi-ming;ZHANG Dong-sheng;WU Hai-wei(Department of Oral and Maxillofacial Surgery,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021;Department of Stomatology,Affiliated Hospital of Heze Medical College,Heze 274000,Shandong Province,China)

机构地区：[1]山东第一医科大学附属省立医院口腔颌面外科,山东东济南250021 [2]菏泽医学专科学校附属医院口腔科,山东菏泽274000

出　　处：《上海口腔医学》2022年第6期576-580,共5页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81901022);山东省自然科学基金(ZR2021MH353)。

摘　　要：目的:探讨紫草素(shikonin,SKN)对血管瘤内皮细胞(hemangioma endothelial cell,HemEC)增殖、凋亡、迁移和血管生成的影响。方法:采用CCK-8及EdU实验,检测SKN对HemEC细胞增殖的影响。流式细胞术检测SKN对HemEC细胞凋亡的影响,采用细胞划痕实验检测SKN对HemEC细胞迁移能力的影响,通过管腔形成实验检测SKN对HemEC细胞血管生成能力的影响。采用SPSS 22.0软件包对数据进行统计学分析。结果:SKN抑制HemEC细胞增殖(P<0.001),促进其凋亡(P<0.001),呈浓度依赖性。SKN抑制HemEC细胞迁移(P<0.01)和血管生成能力(P<0.001)。结论:SKN抑制HemEC细胞增殖、迁移和血管生成,并促进其凋亡。PURPOSE:To investigate the effect of shikonin(SKN) on proliferation,apoptosis,migration and angiogenesis of hemangioma endothelial cell(HemEC).METHODS:CCK-8 and EdU assays were used to detect the effect of SKN on proliferation of HemEC.The effect of SKN on apoptosis of HemEC was detected by flow cytometry.Wound healing assay was used to detect the effect of SKN on migration ability of HemEC.The effect of SKN on angiogenesis ability of HemEC was detected by tube formation assay.SPSS 22.0 software package was used for statistical analysis of the data.RESULTS:SKN inhibited proliferation(P<0.001) and promoted apoptosis(P<0.001) of HemEC in a concentration-dependent manner.In additon,SKN inhibited HemEC migration(P<0.01) and angiogenesis(P<0.001).CONCLUSIONS:SKN can inhibit proliferation,migration,angiogenesis and promote apoptosis of HemEC.

关 键 词：紫草素 血管瘤 血管瘤内皮细胞 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]