80例牙周炎患者牙龈组织中乙型细环病毒和EB病毒载量分析  

Prevalence of torque teno mini virus(TTMV) and epstein-barr virus(EBV) in gingival tissues in 80 patients with periodontitis

作　　者：于阗 张羽[2] 潘少坤[3] 杨彬 冯希平 YU Tian;ZHANG Yu;PAN Shao-kun;YANG Bin;FENG Xi-ping(Department of Preventive Dentistry,Shanghai Stomatological Hospital,Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases,Fudan University,Shanghai 200001;Department of Preventive Dentistry,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai Research Institute of Stomatology,Shanghai 200011;Shanghai Institute of Infectious Disease and Biosecurity,Fudan University,Shanghai 200032,China)

机构地区：[1]上海市口腔医院口腔预防科上海市颅颌面发育与疾病重点实验室,上海200001 [2]上海交通大学医学院附属第九人民医院口腔预防科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011 [3]上海市重大传染病和生物安全研究院,上海200032

出　　处：《上海口腔医学》2022年第6期632-637,共6页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(81800967,81470737);上海市卫生健康委员会科研项目(202140265);上海市口腔医院口腔病防治院临床研究创新团队建设项目(SSDC-2020-CXTD-B01)。

摘　　要：目的:研究80例牙周炎患者牙龈组织中乙型细环病毒(TTMV)和EB病毒(EBV)检出率和病毒载量。方法:收集80例牙周炎和40例牙周健康受试者的牙龈组织样品,运用巢式PCR检测牙龈组织内EBV和TTMV-222病毒的检出率,运用实时荧光定量PCR检测牙龈组织中2种病毒的载量。采用SPSS 16.0软件包对数据进行统计学分析。结果:牙周炎组的EBV和TTMV-222病毒检出率和病毒载量显著高于牙周健康组(P<0.05),且TTMV-222病毒在EBV阳性人群的检出率高于EBV阴性人群(P<0.01),EBV和TTMV-222在牙龈组织内的病毒载量呈正相关(P<0.01)。结论:TTMV感染以及EBV和TTMV的共感染可能与牙周病相关,但2种病毒相互作用的相关机制需要进一步研究。PURPOSE:To study the prevalence of torque teno mini virus(TTMV) and epstein-barr virus(EBV) in patients with periodontitis.METHODS:Gingival tissue samples were collected from 80 patients with periodontitis and 40 periodontal healthy volunteers.The presence of EBV and TTMV-222 were detected by nested PCR,and the virus loads were detected by real-time PCR.Statistical analysis was performed by SPSS 16.0 software package.RESULTS:The detection rates and virus loads of EBV and TTMV-222 in periodontitis group were significantly higher than those in periodontal health group(P<0.05),and the detection rate of TTMV-222 in EBV positive group was significantly higher than that in EBV negative group(P<0.01).There was a positive correlation between EBV and TTMV-222 in gingival tissues(P<0.01).CONCLUSIONS:TTMV infection and co-infection of EBV and TTMV may be related to periodontal disease,but the pathogenic mechanism of the interaction between the two viruses needs further studies.

关 键 词：牙周炎 乙型细环病毒 EB病毒 

分 类 号：R788[医药卫生—口腔医学]