A useful quantitative model for determination of enantiomeric composition of racemate praziquantel by ultraviolet spectroscopy combined with partial least squares and its application to praziquantel tablets  

作　　者：Man Zhao Ran Meng Yifang Lu Lingyun Hu Na Sun Lei Nie Haina Wang 

机构地区：[1]School of Pharmaceutical Sciences,Shandong University Jinan 250012,P.R.China [2]Shandong Cancer Hospital A±liated to Shandong University Jinan,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2018年第3期46-53,共8页创新光学健康科学杂志（英文）

基　　金：supported by National Natural Science Foundation of China(No.81102504);Key Technologies R&D Program of Shandong Province(No.2016GSF201003);the Scienti¯c Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry(the 50th Batch).

摘　　要：A simple and novel method has been proposed to determine the enantiomeric composition ofracemate praziquantel(PzQ)by using the analysis of ultraviolet(UV)spectroscopy combinedwith partial least squares(PLS),This method does not rely on the use of expensive carbohydratessuch as cyclodextrins,but on the use of inexpensive sucrose,which is equally effective as car-bohydrate.PZQ has two enantiomers.Through measuring the slight diference in the UV spectralabsorption of PzQ due to different interactions between its two enantiomers and sucrose,theenantiomeric composition was determined by a quantitative model based on PLS analysis.Themodel showed that the correlation coeficients of calibration set and validation set were 0.9971and 0.9972,respectively.The root mean square error of calibration(RMSEC)and the root meansquare error of prediction(RMSEP)were 0.0167 and 0.0129,respectively,Then,the independentdata of PZQ tablets were also used to test how well the quantitative model of PLS predicted theenantiomeric composition,The ratio of S-PZQ in tablet was 0.492,determined by high-perfor-mance liquid chromatography as the reference value,Six solutions of the tablet samples were prepared,and the ratios of S-PZQ in tablet samples in the validation set were predicted by the PLS model.,Theirrelative erors with the reference value were not more than 4%.Therefore,the established model couldbe accurate and employed to predict the enantiomeric compositions of PzQ tablets.

关 键 词：Enantiomeric compositions PRAZIQUANTEL partia last squares utraviolet spectrscopy SUCROSE 

分 类 号：O62[理学—有机化学]