姜黄素联合有氧运动对高脂饮食大鼠肠道菌群调节作用研究  

COMBINED EFFECTS OF CURCUMIN AND AEROBIC EXERCISE ON INTESTINAL MICROFLORA IN RATS FED A HIGH FAT DIET

作　　者：王丽 吉喆 周海涛[2,3] 董丽 曹建民 WANG Li;JI Zhe;ZHOU Hai-tao;DONG Li;CAO Jian-min(Beijing Sport University,Beijing 100084;Beijing Union University,Beijing 100023;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University,Beijing100191,China)

机构地区：[1]北京体育大学,运动人体科学学院,北京100084 [2]北京联合大学生物化学工程学院,北京100023 [3]北京联合大学生物活性物质与功能食品北京市重点实验室,北京100191

出　　处：《营养学报》2022年第5期498-504,共7页Acta Nutrimenta Sinica

基　　金：北京体育大学2020年中央高校基本科研业务费专项资金资助(No.2020033);北京联合大学科研项目(No.ZK30202104)。

摘　　要：目的本研究将姜黄素与有氧运动联合,用于调节高脂饮食大鼠肠道菌群。方法本研究按照随机分组原则,将40只SD野生雄性大鼠分为普通饮食+安静组(C组)、高脂饮食+安静组(H组)、高脂饮食+姜黄素+安静组(J组)、高脂饮食+有氧运动组(S组)和高脂饮食+姜黄素+有氧运动组(U组),共5组,每组8只。有氧运动方案为:坡度:5°,运动强度:70%最大摄氧量,每次运动60min,每周6次;姜黄素补剂量为200mg/(kg·d),灌胃时间为运动前0.5h。整个实验周期为8w,最后一次有氧运动结束后24h,取盲肠内容物进行测试。结果(1)与H组相比,姜黄素干预、姜黄素联合有氧运动干预增加了高脂饮食大鼠与C组的OTU组成的相似性。(2)在肠道菌群结构分析中发现:与H组相比,J组、S组、U组大鼠肠道菌群中F/B比值发生变化,并且出现不同程度的下降,其中J组、S组大鼠肠道菌群中F/B比值显著性减少(P<0.05),U组大鼠肠道菌群中F/B比值无显著性变化(P>0.05);与U组比较,J组、S组大鼠肠道菌群F/B比值没有显著性变化(P>0.05)。(3)Beta多样性分析中,主要“元素”在三个主坐标中的贡献率分别是43.85%、26.13%、31.14%。(4)ANOSIM相似度分析证实了干预对肠道菌群的有效性(P<0.01)。(5)LEfSe分析中发现C组、H组、J组、S组、U组起重要作用的生物标识分别有13个、10个、6个、1个、11个。结论单一有氧运动或姜黄素干预均可改善高脂饮食大鼠肠道菌群的多样性;二者联合使用的效果并未优于单一干预效果。[营养学报,2022,44(5):498-504]Objective To explore the combined effects of aerobic exercise and curcumin on intestinal microflora in rats fed a high fat diet. Method Forty wild male SD rats were randomly divided into 5 groups: normal diet+quiet group(group C), high-fat diet + quiet(group H), high-fat diet + curcumin + quiet(group J), high-fat diet + aerobic exercise(group S), and high-fat diet + curcumin + aerobic exercise(group U). The aerobic exercise program was as follows: Slope: 5°,exercise intensity: 70% VO2 Max, exercise for 60 minutes each time, 6 times a week. The curcumin was gavaged at dose of 200 mg/(kg bw per·d), and 30 minutes prior to exercise. The whole experiment lasted for 8 weeks, and the cecal contents were collected 24 hours after the last aerobic exercise for gut microflora analysis. Results(1)Compared with group H,curcumin intervention, aerobic exercise combined with curcumin intervention increased the similarity of OTU composition between groups C and H rats.(2)The structure of intestinal flora analysis showed that compared with group H, the F/B ratio of the intestinal flora was changed, significantly in groups J, S and U. Compared with group U, the F/B ratio of the intestinal flora in groups J and S did not significantly change(P>0.05).(3)In beta-diversity analysis, the contribution rates of major "elements" in the three principal coordinates were 43.85%, 26.13% and 31.14%, respectively.(4) ANOSIM similarity analysis confirmed the efficacy of the intervention on intestinal flora(P<0.01).(5)LEfSe analysis found that there were 13,10, 6, 1 and 11 important biomarkers in groups C, H, J, S and U, respectively. Conclusion Both aerobic exercise and curcumin intervention can improve the diversity of intestinal microflora in rats fed a high fat diet. The combination of the two was treatments not more efficaciousthan either of them alone. [ACTA NUTRIMENTA SINICA, 2022,44(5):498-504]

关 键 词：有氧运动 姜黄素 高脂饮食 肠道菌群 

分 类 号：G804.7[文化科学—运动人体科学]