G蛋白偶联受体相关分选蛋白1对三阴性乳腺癌MDA-MB-231细胞生物学功能的影响  

Effects of G protein coupled receptor associated sorting protein 1 on proliferation and apoptosis of triple negative breast cancer MDA-MB-231 cells

作　　者：王丽华[1] 陈海珍[1] 冯女[2] 吴海霞 WANG Lihua;CHEN Haizhen;FENG Nv;WU Haixia(Department of Breast and Thyroid Surgery,Haikou People's Hospital,Haikou,Hainan 570208,China;Central Laboratory of Haikou People's Hospital,Haikou,Hainan 570208,China;Department of Rehabilitation Medicine,Haikou People's Hospital,Haikou,Hainan 570208,China)

机构地区：[1]海口市人民医院乳腺甲状腺外科,海南海口570208 [2]海口市人民医院中心实验室,海南海口570208 [3]海口市人民医院康复医学科,海南海口570208

出　　处：《中国优生与遗传杂志》2022年第12期2135-2139,共5页Chinese Journal of Birth Health & Heredity

摘　　要：目的 探讨G蛋白偶联受体相关分选蛋白1(GASP-1)对三阴性乳腺癌MDA-MB-231细胞生物学功能的影响。方法 将乳腺癌MDA-MB-231细胞分别三组,分别为空白对照组(转染shNC)、shRNA组(转染shRNA)和shGASP组(转染shGASP-1)。qRT-PCR检测GASP-1 mRNA水平,Western blot检测GASP-1蛋白水平,CCK-8检测MDA-MB-231细胞增殖;克隆形成实验检测MDA-MB-231细胞克隆形成能力,Transwell法检测MDA-MB-231细胞迁移能力,流式细胞仪检测MDA-MB-231细胞凋亡。结果 三阴性乳腺癌组织中GASP-1mRNA的表达明显高于癌旁组织(P<0.05)。与空白对照组和shRNA组相比,shGASP组中的GASP-1 mRNA和蛋白显著降低(P<0.05);48~72 h,shGASP组中MDA-MB-231细胞增殖能力显著低于空白对照组(P<0.05);与空白对照组和shRNA组相比,shGASP组中的MDA-MB-231细胞克隆形成能力明显降低(P<0.05);shGASP组中的MDA-MB-231细胞侵袭明显降低(P<0.05);shGASP组中MDA-MB-231细胞凋亡率显著升高(P<0.05)。结论 沉默GASP-1可以抑制MDA-MB-231细胞增殖、侵袭,促进其凋亡,提示GASP-1可能与三阴性乳腺癌的发生发展密切相关,有望成为三阴性乳腺癌治疗的新靶点。Objective To investigate the effect of G protein coupled receptor related sorting protein 1(GASP-1) on the proliferation and apoptosis of triple negative breast cancer MDA-MB-231 cells. Methods Breast cancer MDA-MB-231cells were divided into three groups: blank control group(transfected with SHNC), shRNA group(transfected with shRNA)and shGASP group(transfected with sh GASP-1). GASP-1 mRNA level was detected by QRT PCR, GASP-1 protein level was detected by Western blot, and MDA-MB-231 cell proliferation was detected by CCK-8. The clonogenic ability of MDA-MB-231 cells was detected by clonogenic experiment, the migration ability of MDA-MB-231 cells was detected by Transwell method, and the apoptosis of MDA-MB-231 cells was detected by flow cytometry. Results The expression of GASP-1 mRNA in triple negative breast cancer was significantly higher than that in adjacent tissues(P<0.05). Compared with the blank control group and shRNA group, The expression of GASP-1 mRNA and protein in shGASP group decreased significantly(P<0.05). At 48-72 hours, the proliferation of MDA-MB-231 cells in shGASP group was significantly lower than the blank control group(P<0.05). The clonogenic ability of MDA-MB-231 cells in shGASP group was significantly lower than that in blank control group and shRNA group(P<0.05). The invasion of MDA-MB-231 cells in shGASP group decreased significantly(P<0.05). The apoptosis rate of MDA-MB-231 cells in shGASP group increased significantly(P<0.05). Conclusion Silencing GASP-1 can inhibit the proliferation and invasion of MDA-MB-231 cells and promote their apoptosis, suggesting that GASP-1may be closely related to the occurrence and development of triple negative breast cancer, and may become a new target for the treatment of triple negative breast cancer.

关 键 词：G蛋白偶联受体相关分选蛋白1 三阴性乳腺癌 增殖 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]