miR-25-3p靶向调节PTEN对子宫内膜癌细胞增殖、凋亡和迁移侵袭的影响  

The impacts of miR-25-3p on the proliferation, apoptosis, migration and invasion of endometrial cancer cells by targeting and regulating PTEN

作　　者：张秀俊[1] 陈玉芬[1] 朱芳[1] 霍艳[1] 于华[1] 苏海飞[2] ZHANG Junxiu;CHEN Yufen;ZHU Fang;HUO Yan;YU Hua;SU Haifei(Department of Obstetrics,the First Hospital of Handan,Handan,Hebei 056500,China;Department of Obstetrics and Gynecology,Guantao People’s Hospital,Handan,Hebei 057750,China)

机构地区：[1]邯郸市第一医院产科,河北邯郸056500 [2]馆陶县人民医院妇产科,河北邯郸057750

出　　处：《中国优生与遗传杂志》2022年第11期1920-1926,共7页Chinese Journal of Birth Health & Heredity

基　　金：河北省医学科学研究重点课题计划项目(20160332)。

摘　　要：目的探究miR-25-3p靶向调节10号染色体上缺失的磷酸酶与张力蛋白同源物基因(PTEN)对子宫内膜癌(EC)细胞增殖、凋亡和迁移侵袭的影响。方法qRT-PCR检测人子宫内膜上皮细胞与人EC细胞株HEC-1-A、RL95-2、Ishikawa中miR-25-3p、PTEN表达。取Ishikawa细胞随机分为对照组、miR-25-3p inhibitor组、miR-25-3p inhibitor阴性对照+空载组、共转染组,分组转染后,检测各组Ishikawa细胞增殖、凋亡、迁移、侵袭、增殖蛋白(PCNA)和凋亡蛋白(caspase-9、Bax)、上皮间质转化标志蛋白(Vimentin、E-cadherin)表达、miR-25-3p和PTEN mRNA表达、miR-25-3p对PTEN的靶向调控作用。结果与人子宫内膜上皮细胞相比,人EC细胞株Ishikawa、HEC-1-A、RL95-2中miR-25-3p表达升高(P<0.05),PTEN mRNA表达降低(P<0.05)。与对照组相比,miR-25-3p inhibitor组细胞迁移距离、侵袭细胞数、细胞miR-25-3p及蛋白PCNA、Vimentin表达均降低(P<0.05),增殖抑制率、凋亡率、细胞PTEN mRNA及蛋白caspase-9、Bax、E-cadherin表达均升高(P<0.05)。结论敲低miR-25-3p可通过增强PTEN表达而抑制EC细胞增殖和上皮间质转化,降低其迁移侵袭能力,并诱导其凋亡。Objective To explore the impacts of miR-25-3p on the proliferation,apoptosis,migration and invasion of endometrial cancer(EC)cells by targeting the regulation of phosphatase and tensin homolog deleted on chromosome ten(PTEN).Methods qRT-PCR was performed to detect the expression of miR-25-3p and PTEN in human endometrial epithelial cells and human EC cell lines HEC-1-A,RL95-2,Ishikawa.Ishikawa cells were randomly separated into control group,miR-25-3p inhibitor group,and miR-25-3p inhibitor negative control+empty group,and co-transfection group.After grouping and transfection,the proliferation,apoptosis,migration,invasion,the expression of proliferation protein(PCNA)and apoptosis proteins(caspase-9,Bax),epithelial-mesenchymal transition marker proteins(Vimentin,E-cadherin),the expression of miR-25-3p and PTEN mRNA,the targeted regulation of miR-25-3p on PTEN of Ishikawa cells in each group were detected.Results Compared with human endometrial epithelial cells,the expression of miR-25-3p in human EC cell lines Ishikawa,HEC-1-A and RL95-2 was increased(P<0.05),the expression of PTEN mRNA was decreased(P<0.05).Compared with the control group,the migration distance of cells,the number of invasive cells,the expression of miR-25-3p,the PCNA and Vimentin proteins in the miR-25-3p inhibitor group were all decreased(P<0.05),the proliferation inhibition rate,apoptosis rate,and the expression of PTEN mRNA and caspase-9,Bax,and E-cadherin proteins were all increased(P<0.05).Conclusion Knockdown of miR-25-3p can inhibit the proliferation and epithelial-mesenchymal transition of EC cells by enhancing the expression of PTEN,reduce their migration and invasion ability,and induce their apoptosis.

关 键 词：miR-25-3p 张力蛋白同源物基因 子宫内膜癌 增殖 

分 类 号：R737.33[医药卫生—肿瘤]