棒状腐败乳杆菌Lc7 的生物学特性及益生作用  

Biological characteristics and probiotic effect of Loigolactobacilluscoryniformis Lc7

作　　者：张素平 林晓颖 徐明超 乔蕾 杨晶[2,3] 孙晖 张桂[2] 刘丽云[2,3] 徐建国[1,2,3] ZHANG Suping;LIN Xiaoying;XU Mingchao;QIAO Lei;YANG Jing;SUN Hui;ZHANG Gui;LIU Liyun;XU Jianguo(School of Public Health,Nanjing Medical University,Nanjing 211166,Jiangsu,China;State Key Laboratory for Communicable Disease Prevention and Control,National Institute for Communicable Disease Prevention and Control,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Research Units of Discovery of Unknown Bacteria and Function,Chinese Academy of Medical Sciences,Beijing 102206,China)

机构地区：[1]南京医科大学公共卫生学院,江苏南京211166 [2]中国疾病预防控制中心传染病预防控制所、传染病预防控制国家重点实验室,北京102206 [3]中国医学科学学院未知细菌发现和功能研究创新单元,北京102206

出　　处：《微生物学报》2023年第1期319-332,共14页Acta Microbiologica Sinica

基　　金：国家重点研发计划(2019YFC1200505);未知微生物发现和功能研究(2018RU010)。

摘　　要：【目的】对分离自健康成人粪便样本的棒状腐败乳杆菌(Loigolactobacillus coryniformis)Lc7进行分类学鉴定和益生潜力评估。【方法】基于16S rRNA基因和基因组核心基因构建系统发育树,对Lc7进行分类学鉴定;通过耐酸和胆汁酸盐、粘附、抗氧化和抑菌实验,以及溶血、明胶酶活性和抗菌药物敏感性实验,评估Lc7的益生特性。同时,构建小鼠溃疡性结肠炎模型,评估Lc7的体内抗炎潜力。【结果】Lc7鉴定为L.coryniformis,在酸和胆汁酸盐的连续作用下,Lc7的存活率为70.17%。Lc7对HT-29细胞的粘附指数为56.33 CFU/cell,其自聚集和疏水性分别为80%和40%;Lc7对福氏志贺菌和鼠伤寒沙门菌等7个常见致病菌均有较强的抑制能力;对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picryl-hydrazyl,DPPH)和羟自由基(hydroxyl radicals,·OH)的清除率分别为91.70%和48.53%;Lc7无溶血现象和明胶酶活性,对选取的大多数抗生素均敏感。在小鼠结肠炎实验中,Lc7干预组小鼠结肠长度明显长于模型组(P<0.01),结肠病理明显改善(P<0.01),并且降低了血清促炎细胞因子TNF-α和IL-1β的浓度,升高了抗炎细胞因子IL-10的浓度(P<0.01)。【结论】Lc7具有益生特性和体内抗炎潜力,可作为益生菌株进行开发和应用。[Objective]To characterize the Loigolactobacillus coryniformis Lc7 isolated from feces of healthy adults and evaluate its probiotic effect.[Methods]Phylogenetic tree was constructed based on the 16S rRNA gene and the core genes of the genome for taxonomic identification of the strain.The tolerance to acid,bile salt tolerance,adhesion,antioxidant activity,and antibacterial activity were tested,and the hemolysis,gelatinase activity,and antimicrobial susceptibility of Lc7 were examined to evaluate the probiotic property.In addition,ulcerative colitis was induced in mice to assess the in vivo anti-inflammatory potential of the strain.[Results]Lc7 was identified as L.coryniformis.The survival rate of this strain was up to 70.17%at pH 3.0 and in the presence of 0.3%bile salt.The adhesion index of Lc7 to HT-29 cells was 56.33 CFU/cell while self-aggregation and hydrophobicity were 80%and 40%,respectively.Lc7 inhibited the growth of seven common pathogens including Shigella flexneri and Salmonella typhimurium.Moreover,it scavenged 91.70%and 48.53%of 1,1-diphenyl-2-picryl-hydrazyl(DPPH)and hydroxyl radicals(·OH),respectively.Lc7 showed no hemolysis or gelatinase activity and was sensitive to the antibiotics tested.In the experiment on colitis mice,Lc7 group had longer colons than the model group(P<0.01).In addition,Lc7 alleviated colonic lesions(P<0.01),decreased serum pro-inflammatory cytokines TNF-αand IL-1β,and increased serum anti-inflammatory cytokines IL-10(P<0.01).[Conclusion]Lc7 has probiotic and anti-inflammatory properties,which can be further developed as a probiotic.

关 键 词：棒状腐败乳杆菌 分类学鉴定 益生菌 抗炎 

分 类 号：TS201.3[轻工技术与工程—食品科学]