宫颈癌组织中NEK2和circPITX1的表达水平与放疗敏感度及预后的相关性研究  

Correlation between the Expression Levels of NEK2 and CircPITX1 and Radiotherapy Sensitivity and Prognosis in Cervical Cancer

作　　者：陈霄 吴小颖 安明 CHEN Xiao;WU Xiao-ying;AN Ming(Department of Obstetrics and Gynecology,West China Sanya Hospital of Sichuan University,Hainan Sanya 572000,China)

机构地区：[1]四川大学华西三亚医院妇产科,海南三亚572000

出　　处：《现代检验医学杂志》2023年第1期27-32,共6页Journal of Modern Laboratory Medicine

基　　金：海南省自然科学基金项目(20169426)。

摘　　要：目的探讨宫颈癌组织中中心体相关激酶2(never in mitosis geneA related kinase-2,NEK2)和环状RNA配对同源结构域基因1(circRNA paired homology domain gene 1,circPITX1)表达与放疗敏感度及预后的关系。方法选取2017年1月~2018年1月四川大学华西三亚医院接受放疗治疗的82例宫颈癌患者为研究对象。依据放疗后病灶退缩情况评价放疗疗效,分为放疗抵抗组(n=51)和放疗敏感组(n=31)。应用免疫组织化学法(immunohistochemistry,IHCS)检测宫颈癌癌组织和癌旁组织中NEK2蛋白表达。采用荧光实时定量PCR(real-time quantitative PCR,RT-qPCR)检测circPITX1mRNA和NEK2mRNA表达。统计学分析癌组织中circPITX1mRNA和NEK2 mRNA与放疗敏感度、临床病理特征的关系。采用KaplanMeier生存曲线分析宫颈癌患者circPITX1mRNA和NEK2 mRNA表达水平与患者生存预后的关系。COX回归分析宫颈癌患者预后不良的危险因素。结果宫颈癌癌组织中NEK2蛋白表达阳性率[85.37%(70/82)]高于癌旁组织[12.20%(10/82)],差异有统计学意义(χ^(2)=87.857,P<0.05)。癌组织中circPITX1mRNA(1.92±0.24),NEK2 mRNA表达(2.04±0.31)高于癌旁组织(0.81±0.20,0.93±0.27)表达,差异均有统计学意义(t=32.174,24.450,均P<0.05)。宫颈癌组织中NEK2 mRNA与circPITX1mRNA表达呈显著正相关(r=0.618,P<0.05)。FIGO分期ⅢA~ⅢB期宫颈癌患者NEK2 mRNA(2.54±0.23),circPITX1mRNA(2.33±0.19)表达明显高于IIB期患者(1.17±0.40,1.21±0.30)表达,差异均有统计学意义(t=19.730,20.710,均P<0.05)。宫颈癌放疗抵抗组NEK2 mRNA(2.39±0.38),circPITX1mRNA(2.50±0.21)均显著高于放疗敏感组(1.83±0.28,0.97±0.28),差异有统计学意义(t=7.109,28.149,均P<0.05)。NEK2 mRNA[(48.78%(20/41)]和circPITX1mRNA[54.76%(23/42)]高表达组的三年总生存率显著低于NEK2 mRNA[90.24%(37/41)],circPITX1 mRNA低表达组[85.00%(34/40)],差异均有统计学意义(χ^(2)=5.118,4.890,均P<0.05);NEK2 mRNA[(26.10±3.62)个月]和circPITX1mRNA[(25.87±3.81)个月]高表�Objective To explore the expression never in mitosis gene A-related kinase 2(NEK2)and circRNA paired homology domain gene 1(circPITX1)in cervical cancer tissues and their correlation with radiotherapy sensitivity and prognosis.Methods 82 patients with cervical cancer who received radiotherapy in West China Sanya Hospital of Sichuan University from January 2017 to January 2018 were selected as research object.The efficacy of radiotherapy was evaluated according to the lesion shrinkage after radiotherapy,and the patients were divided into radiotherapy resistant group(n=51)and radiotherapy sensitive group(n=31).The expression of NEK2 protein in cervical cancer tissues and adjacent tissues was detected by immunohistochemistry(iIHCS).The expression of circPITX1mRNA and NEK2 mRNA was detected by fluorescence realtime quantitative PCR(RT-qPCR).The relationship between circPITX1 mRNA and NEK2 mRNA in cancer tissue with radiosensitivity and clinicopathological characteristics was statistically analyzed.The Kaplan-Meier survival curve was used to analyze the relationship between the expression levels of circPITX1mRNA and NEK2 mRNA and the 3-year prognosis of cervical cancer patients.COX regression analysis was used to analyze the risk factors of poor prognosis in patients with cervical cancer.Results The positive rate of NEK2 protein expression in cervical cancer tissues[85.37%(70/82)]was higher than that in adjacent tissues[12.20%(10/82)],and the difference was statistically significant(χ^(2)=87.857,P<0.05).The expressions of circPITX1mRNA(1.92±0.24)and NEK2 mRNA(2.04±0.31)in cancer tissues were higher than those of circPITX1 mRNA(0.81±0.20)and NEK2 mRNA(0.93±0.27)paracancerous tissues,and the differences were statistically significant(t=32.174,24.450,all P<0.05).There was a significant positive correlation between NEK2 mRNA and CircPITX1mRNA expression in cervical cancer tissue(r=0.618,P<0.05).The expressions of NEK2 mRNA(2.54±0.23)and circPITX1mRNA(2.33±0.19)in patients with FIGO stage IIIA~IIIB cervical cancer w

关 键 词：宫颈癌 中心体相关激酶2 环状RNA配对同源结构域基因1 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]