亚铁离子荧光探针研究进展  

Research Progress of Ferrous Ion Fluorescent Probe

作　　者：李佳佳 张瑞龙[1] 张忠平 LI Jiajia;ZHANG Ruilong;ZHANG Zhongping(School of Chemistry and Chemical Engineering,Anhui University,Hefei 230601,China;Institute of Physical Science and Information Technology,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学化学化工学院,合肥230601 [2]安徽大学物质科学与信息技术研究院,合肥230601

出　　处：《材料导报》2023年第2期222-229,共8页Materials Reports

基　　金：国家自然科学基金(21705001;21775001)。

摘　　要：铁作为生物体内最丰富的过渡金属元素,与生命体的新陈代谢息息相关,而且铁元素主要以亚铁离子(Fe^(2+))的形式存在于细胞内。体内Fe^(2+)含量的失调与贫血症、癌症和心血管疾病等多种病症的发生和发展有着直接的联系。因此,监测生命体内Fe^(2+)的浓度是评估人体健康状况的重要手段。在诸多Fe^(2+)的检测方法中,荧光检测法因操作快速、简便并可用于体内/体外实时监测等最具发展前景。然而,在荧光检测法中探针的设计与筛选至关重要。传统的荧光探针面临的最大问题是特异性,即对Fe^(2+)的响应常常会受到其他金属离子(如钙离子、镁离子和钾离子等)的干扰。因此,为了适用于监测生物系统中Fe^(2+)浓度,设计出特异性强、光稳定性好、灵敏度高、检测限低和生物相容性优良的荧光探针具有重要的意义。现阶段,Fe^(2+)荧光探针主要分为两大类:有机小分子和纳米材料,其机理主要可分为三种:(1)还原N-O类化合物;(2)与Fe^(2+)螯合;(3)利用Fe^(2+)与荧光探针发生特异性反应(如Fenton反应、选择性断裂酰胺或羟胺键等)。本文主要归纳总结了近十几年Fe^(2+)荧光探针的研究进展,包括制备方法、作用机理和在生命系统中检测Fe^(2+)的应用进展。最后,对如何进一步优化Fe^(2+)荧光探针的发光性质、检测限和特异性提出了展望。Ferrous ion(Fe^(2+)),as the most abundant iron element in living organisms,is closely related to the metabolism in live organisms.The abnor-mal Fe^(2+) levels involve in the occurrence and development of various diseases such as anemia,cancer and cardiovascular diseases.Therefore,the monitoring of Fe^(2+) fluctuation in living systems is of great significance to evaluate human health.Among of the several Fe^(2+) detection approaches,fluorescence method is widely employed due to its easy operation and on-site capability in vivo/in vitro.Design and screening of fluorescent probes play key roles in fluorescence detection.However,a typical difficulty of the traditional fluorescent probes is how to avoid the interference from other metal ions(such as calcium,magnesium and potassium).Thus,the designs of fluorescent probes with excellent specificity,photostability,sensitivity and biocompatibility have been devoted by scientists.According the materials of probes,they are always classified into organic molecular probes and nano probes.In parallel,the probes included three reaction mechanisms for detecting Fe^(2+):(1)reduction of NO groups;(2)chelation with Fe^(2+);(3)specifical reaction with Fe^(2+)(for example:Fenton reaction,selective cleavage of amide or hydroxylamine bonds,etc.).Here,we have summarized the research progress of Fe^(2+) fluorescent probes in the past decade,including synthesis methods,detection mechanism and bio-application progress.We envision the further optimization of the luminescence property,detection limit and specificity of fluorescent probe.

关 键 词：亚铁离子 荧光探针 识别 生物成像 

分 类 号：O657.3[理学—分析化学]