一株碳酸盐矿化菌的筛选及其矿化性能研究  

Screening of a Carbonate Mineralized Strain and its Mineralized Properties

作　　者：胡南[1,2] 李艾书 仇晓钰 刘晶晶 王新廷[1,2] 张辉 李广悦[1,2] 丁德馨[1,2] HU Nan;LI Aishu;QIU Xiaoyu;LIU Jingjing;WANG Xinting;ZHANG Hui;LI Guangyue;DING Dexin(Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy,University of South China,Hengyang,Hunan 421001,China;Hunan Province Key Laboratory of Green Development Technology for Extremely Low Grade Uranium Resources,University of South China,Hengyang,Hunan 421001,China)

机构地区：[1]南华大学铀矿冶生物技术国防重点学科实验室,湖南衡阳421001 [2]南华大学极贫铀资源绿色开发技术湖南省重点实验室,湖南衡阳421001

出　　处：《南华大学学报（自然科学版）》2022年第5期1-6,47,共7页Journal of University of South China：Science and Technology

基　　金：国家自然科学基金资助项目(U20A20267);湖南省杰出青年基金项目(2022JJ10041);湖南省科技人才托举项目(2020TJ-Q03);湖南省教育厅重点资助项目(19A436);湖南省重点研发计划项目(2022SK2077)。

摘　　要：采用选择性富集和平板分离的方法从湖南省某土壤中筛出一株以乙酸钙为底物的碳酸盐矿化菌,并对该菌株诱导的碳酸钙沉淀的晶体类型以及pH、接种量、乙酸钙浓度等不同因素对碳酸钙的产量影响进行研究。结果表明,该碳酸盐矿化菌鉴定为克雷白氏杆菌属,其GenBank登录号为ON139648;革兰氏染色呈阴性,6 h~96 h为该菌的对数生长期;克雷白氏杆菌诱导的碳酸钙矿物晶体类型既有方解石,又有球霰石,其诱导碳酸钙沉淀产生的最佳条件为:初始pH=7、菌液接种量为4%、乙酸钙浓度为0.25 mol/L,乙酸钙利用率最高为96.6%,诱导的碳酸钙产量最高为1.23 g。A strain of calcium carbonate mineralization bacteria based on calcium acetate as substrate was isolated from a soil in Hunan Province by selective enrichment and plate separation,and the crystal type of calcium carbonate precipitation induced by this strain and the effects of different factors such as pH,inoculum and calcium acetate concentration on calcium carbonate production were investigated.The results showed that the carbonate mineralizing bacteria were identified as Klebsiella sp.with GenBank accession number ON139648;the Gram stain was negative,and the logarithmic growth period was from 6 h to 96 h.The types of calcium carbonate crystals induced by Klebsiella sp.were both calcite and vaterite,and the optimal conditions for inducing calcium carbonate precipitation were:initial pH=7,inoculum level of 4%,calcium acetate concentration of 0.25 mol/L,maximum calcium acetate utilization of 96.6%,and maximum induced calcium carbonate yield of 1.23 g.

关 键 词：微生物诱导碳酸钙沉淀 乙酸钙 克雷白氏杆菌 

分 类 号：X172[环境科学与工程—环境科学]