下调S100A2表达对结肠癌细胞增殖、侵袭迁移和Wnt信号通路的影响  

Effects of S100A2 down-regulation on colon cancer cells’ proliferation, invasion, migration and Wnt signaling pathway

作　　者：龚国金 殷朝丽 冯泽勇 吴洪俊 邓翔 GONG Guojin;YIN Chaoli;FENG Zeyong;WU Hongjun;DENG Xiang(Department of Gastrointestinal Surgery,Xichang People's Hospital,Xichang 615000,China)

机构地区：[1]西昌市人民医院胃肠外科,四川西昌615000 [2]西昌市人民医院消化内科,615000

出　　处：《临床肿瘤学杂志》2022年第12期1099-1104,共6页Chinese Clinical Oncology

摘　　要：目的 探讨S100钙结合蛋白A2(S100A2)在结肠癌增殖和侵袭迁移中的作用及可能机制。方法 通过实时荧光定量PCR(qPCR)检测S100A2在结肠癌组织和细胞系中的表达情况。选择SW480细胞并分为3组:对照组、siRNA-NC组(阴性对照)和siRNA-S100A2组(下调S100A2表达)。采用MTT、划痕实验和Transwell小室实验探究S100A2在结肠癌细胞增殖、侵袭和迁移过程中的作用,qPCR和Western blotting检测干扰S100A2对Wnt1/β-连环蛋白(β-catenin)信号通路的影响。结果 与癌旁组织(1.00±0.05)或正常结肠上皮细胞株NCM460(1.00±0.07)比较,S100A2在结肠癌组织(1.66±0.07)和细胞SW620(1.38±0.10)、HT29(1.62±0.13)、LoVo(1.94±0.14)和SW480(2.03±0.08)中均高表达,差异有统计学意义(P<0.05)。siRNA-S100A2组的细胞活力及Wnt1、β-catenin和基质金属蛋白酶7水平低于对照组和siRNA-NC组(P<0.05);siRNA-S100A2组细胞划痕愈合率和穿膜细胞数为(34.94±3.11)%和(115.65±15.24)个,少于siRNA-NC组的(80.66±5.86)%和(358.88±24.60)个及对照组的(78.54±4.61)%和(371.39±18.90)个,差异有统计学意义(P<0.05)。结论 S100A2促进结肠癌细胞的增殖和迁移侵袭,可能与Wnt1/β-catenin信号轴的激活有关。Objective To investigate the role of S100 calcium-binding protein A2(S100A2) on the proliferation, invasion and migration of colon cancer cells and its possible mechanism. Methods Expressions of S100A2 in colon cancer tissues and cell lines were detected by real-time fluorescent quantitative PCR(qPCR). SW480 cells were selected and divided into three groups: control group, siRNA-NC group(negative control) and siRNA-S100A2 group(down-regulation of S100A2). MTT, scratch test and Transwell chamber test were used to explore the role of S100A2 in the proliferation, invasion and migration of colon cancer cells.Western blotting and qPCR were applied to evaluate the influence of S100A2 down-regulation on Wnt1/β-catenin pathway. Results Compared with adjacent tissues(1.00±0.05) or normal colon epithelial cell line NCM460(1.00±0.07), S100A2 was highly expressed in colon cancer tissues(1.66±0.07) and colon cancer cells including SW620(1.38±0.10), HT29(1.62±0.13), LoVo(1.94±0.14) and SW480(2.03±0.08), with a statistically significant difference(P<0.05). Cell viability and levels of Wnt1, β-catenin and metalloproteinase 7 were lower in siRNA-NC group than those in control group(P<0.05). In siRNA-S100A2 group, the scratch healing rate and the number of cells penetrating the membrane were(34.94 ± 3.11)% and 115.65±15.24, less than(80.66±5.86)% and 358.88±24.60 in siRNA-NC group and(78.54±4.61)% and 371.39±18.90 in control group with significant differences(P<0.05). Conclusion This study revealed that S100A2 promoted cell proliferation, migration and invasion of colon cancer, which may be related with the activation of Wnt1/β-catenin signaling axis.

关 键 词：结肠癌 S100A2 增殖 侵袭迁移 Wnt1/β-连环蛋白信号通路 

分 类 号：R735.3[医药卫生—肿瘤]