胰腺癌中OASL的表达及其对癌细胞增殖和迁移的影响  

OASL Expression in Pancreatic Cancer and Its Effect on Proliferation and Migration of Pancreatic Cancer Cells

作　　者：张人丹[1] 赵春艳[2] 姚佳欣 胡先华[1] 母波[1] ZHANG Rendan;ZHAO Chunyan;YAO Jiaxin;HU Xianhua;MU Bo(School of Basic Medical Sciences and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,China;Sichuan Key Laboratory of Medical Imaging,North Sichuan Medical College,Nanchong 637000,China)

机构地区：[1]川北医学院基础医学与法医学院,南充637000 [2]川北医学院四川省医学影像重点实验室,南充637000

出　　处：《肿瘤防治研究》2023年第1期18-26,共9页Cancer Research on Prevention and Treatment

基　　金：川北医学院校级科研发展计划项目(CBY21-QA17);南充市市校合作项目(22SXCXTD0002);南充市市校合作项目(22SXQT0100);四川省科技厅苗子工程项目(2021033)。

摘　　要：目的探讨OASL的表达对胰腺癌细胞增殖和迁移能力的影响。方法GEPIA数据库分析OASL在胰腺癌组织和正常胰腺组织中的表达差异。TIMER数据库分析OASL表达与患者生存期的关系。TCGA数据库分析OASL表达与胰腺癌临床病理参数的相关性。shRNA技术敲减胰腺癌panc-1细胞中OASL基因的表达。慢病毒用于过表达胰腺癌panc-1细胞中OASL基因。MTT实验检测胰腺癌panc-1细胞的增殖能力,划痕实验及Transwell实验检测panc-1细胞的迁移能力,Westernblot实验检测与肿瘤增殖、迁移、侵袭相关蛋白的表达。结果胰腺癌组中的OASL表达量显著高于正常胰腺组织(P<0.05),且胰腺癌患者中OASL高表达患者与低表达患者相比具有较差的总生存期(P<0.05)。敲减OASL基因后,panc-1细胞的增殖和迁移能力均被抑制,而过表达OASL基因促进了panc-1细胞的增殖和迁移能力。敲减OASL后,p-STAT3蛋白表达增高,STAT3和BAK蛋白表达降低;过表达OASL后,p-STAT3蛋白表达降低,STAT3和BAK蛋白表达增高。结论OASL可能通过STAT3信号通路影响胰腺癌细胞增殖和迁移能力以及BAK表达来诱导细胞凋亡。Objective To explore the effect of OASL expression on the proliferation and migration of pancreatic cancer cells.Methods The GEPIA database was used to analyze the differences in OASL expression in pancreatic cancer tissues and normal pancreatic tissues.The TIMER database was used to analyze the relationship between OASL expression and patient survival.The TCGA database was used to analyze the correlation of OASL expression with the clinicopathological parameters of pancreatic cancer.shRNA was used to knock down the expression of OASL gene in pancreatic cancer panc-1 cells.Lentiviruses were used to overexpress the OASL gene in pancreatic cancer cells.MTT assay was used to evaluate their proliferation ability,and scratch and Transwell experiments were used to evaluate their migration ability.Western blot experiments were used to detect changes in proteins related to tumor proliferation,migration,and invasion.Results OASL expression in the pancreatic cancer group was significantly higher than that in normal pancreatic tissue(P<0.05),and patients with high OASL expression in pancreatic cancer patients had worse OS than patients with low expression(P<0.05).After OASL gene knockdown,the proliferation and migration abilities of panc-1 cells were inhibited,whereas the overexpression of OASL gene promoted the proliferation and migration ability of panc-1 cells.Western blot experiments showed that after OASL knockdown,p-STAT3 protein expression increased,whereas STAT3 and BAK protein expressions decreased.After OASL overexpression,p-STAT3 protein expression decreased,and STAT3 and BAK protein expression increased.Conclusion OASL may affect the proliferation and migration of pancreatic cancer cells through the STAT3 signaling pathway while affecting BAK expression to induce cell death.

关 键 词：胰腺癌 OASL 生物信息学 

分 类 号：R735.9[医药卫生—肿瘤]