Characteristic analysis of skin keratinocytes in patients with type 2 diabetes based on the single-cell levels  

作　　者：Bingye Liao Qiuyi Ouyang Hongqin Song Ziqi Wang Jinhua Ou Jinxin Huang Liang Liu 

机构地区：[1]Department of Operating Room,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510080,China [2]Wound Treatment Department,Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou,Guangdong 510315,China

出　　处：《Chinese Medical Journal》2022年第20期2461-2466,共6页中华医学杂志（英文版）

摘　　要：Background: Keratinocytes play an important role in wound healing;however, less is known about skin keratinocytes in patients with type 2 diabetes mellitus (T2DM). Therefore, this study aimed to search for the transcriptional characteristics of keratinocytes at the single-cell level from T2DM patients, and to provide experimental data for identifying the pathological mechanisms of keratinocytes under pathological conditions.Methods: We performed single-cell RNA sequencing on the skin tissue from two T2DM patients and one patient without diabetes-induced trauma using the BD Rhapsody^(TM) Single-Cell Analysis System. With the help of bioinformatics R-based single-cell analysis software, we analyzed the results of single-cell sequencing to identify the single-cell subsets and transcriptional characteristics of keratinocytes at the single-cell level, including Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyzes.Results: In this study, we found specific highly expressed signature keratinocyte-related genes. We analyzed the transcriptome of keratinocytes from experimental and control groups and screened a total of 356 differential genes, which were subject to bioinformatics analysis. Enriched pathways included oxidative phosphorylation, antigen processing and presentation, prion and Huntingtons’ diseases, bacterial invasion of epithelial cells, thermogenesis, vasopressin-regulated water reabsorption, and protein processing in the endoplasmic reticulum.Conclusions: This study revealed the characteristics of keratinocytes at the single-cell level and screened a group of differentially expressed genes related to T2DM-associated keratinocytes, oxidative phosphorylation, cytokine receptor interactions, prion diseases, and other signaling pathways.

关 键 词：Diabetes mellitus type 2 Gene Ontology KERATINOCYTES Prion diseases Sequence analysis RNA Single-cell analysis TRANSCRIPTOME Wound healing 

分 类 号：R587.1[医药卫生—内分泌]