Effects of Tuina on serum creatine kinase and skeletal muscle mitochondria in delayed onset muscle soreness model rats  

推拿对延迟性肌肉酸痛模型大鼠血清肌酸激酶及骨骼肌线粒体的影响

作　　者：WEI Qingbo ZHAO Qian GU Jialing LIN Jia ZHU Yan SONG Ziqi LI Fenglei YANG Yanping 韦庆波;赵谦;顾嘉凌;林佳;朱延;宋子琪;李风雷;杨燕萍(Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Second Chinese Medicine Hospital,Nanjing 210017,China;Jiangsu Haibin Rehabilitation Hospital,Lianyungang 222042,China;不详)

机构地区：[1]Nanjing University of Chinese Medicine,Nanjing 210023,China [2]Jiangsu Second Chinese Medicine Hospital,Nanjing 210017,China [3]Jiangsu Haibin Rehabilitation Hospital,Lianyungang 222042,China [4]不详

出　　处：《Journal of Acupuncture and Tuina Science》2022年第6期446-452,共7页针灸推拿医学（英文版）

基　　金：This work was supported by the Science and Technology Project of Jiangsu Bureau of Traditional Chinese Medicine(江苏省中医药局科技项目,No.YB201849).

摘　　要：Objective To observe the effect of Tuina(Chinese therapeutic massage)on creatine kinase(CK),mitochondrial Ca^(2+)concentration,and ultrastructure of skeletal muscle in delayed onset muscle soreness(DOMS)model rats.Methods A total of 130 healthy male Sprague-Dawley rats were randomly divided into a blank group,an exercise control group,a pre-exercise Tuina group,and a post-exercise Tuina group.According to the time points for sample collection,the exercise control group was divided into a 0 h exercise control group,a 24 h exercise control group,a 48 h exercise control group,and a 72 h exercise control group;the pre-exercise Tuina group was further divided into a 0 h pre-exercise Tuina group,a 24 h pre-exercise Tuina group,a 48 h pre-exercise Tuina group,and a 72 h pre-exercise Tuina group;and the post-exercise Tuina group was divided into a 0 h post-exercise Tuina group,a 24 h post-exercise Tuina group,a 48 h post-exercise Tuina group,and a 72 h post-exercise Tuina group.Rats in all groups except for the blank group received DOMS modeling.Professionals performed Nie-Pinching manipulation and finger Nian-Twisting manipulation on the lower limbs of the rats.The samples were collected at 0 h,24 h,48 h,or 72 h after exhaustive exercise for each pre-exercise Tuina group.The samples were collected at 0 h,24 h,48 h,or 72 h after Tuina for each post-exercise Tuina group.The changes in serum CK,skeletal muscle mitochondrial Ca^(2+)concentration,and Ca^(2+)-adenosine triphosphatase(ATPase)were determined.The ultrastructure changes of skeletal muscles in each group were observed by a transmission electron microscope.Results The electron microscope showed that compared with the exercise control group,the skeletal muscle structures of the pre-exercise Tuina group and the post-exercise Tuina group were significantly improved,and the overall performance of skeletal muscle in the pre-exercise Tuina group was more similar to that of the blank group.The level of serum CK in the pre-exercise Tuina group and the post-exercise Tuina g目的:观察推拿对延迟性肌肉酸痛(DOMS)模型大鼠骨骼肌肌酸激酶(CK)、线粒体Ca^(2+)浓度以及骨骼肌超微结构的影响.方法:将130只健康雄性Sprague-Dawley大鼠按随机数字表法分为空白组、运动对照组、运动前推拿组和运动后推拿组.依据取材时间点的不同,运动对照组又分为运动对照0 h组、运动对照24 h组、运动对照48 h组和运动对照72 h组;运动前推拿组又分为运动前推拿0 h组、运动前推拿24 h组、运动前推拿48 h组和运动前推拿72 h组;运动后推拿组又分为运动后推拿0 h组、运动后推拿24 h组、运动后推拿48 h组和运动后推拿72 h组.除空白组外,其余各组大鼠均接受DOMS模型造模.由专业人员在大鼠的两侧下肢施以捏法和捻法,运动前推拿的各组分别在力竭运动后0 h、24 h、48 h及72 h进行取材,运动后推拿的各组分别在推拿操作后0 h、24 h、48 h及72 h进行取材,测定大鼠血清CK、骨骼肌线粒体Ca^(2+)浓度、Ca^(2+)-三磷酸腺苷酶(ATPase)的变化;通过透射电镜观察各组大鼠骨骼肌超微结构的改变.结果:电镜观察结果表明,与运动对照组相比,运动前推拿组及运动后推拿组的骨骼肌结构有明显改善,运动前推拿组骨骼肌整体表现与空白组更接近;运动前推拿组和运动后推拿组各时段血清CK含量均较运动对照组显著减少(P<0.01);运动前推拿24 h、48 h及72 h组骨骼肌Ca^(2+)浓度较同时段运动后推拿组减少(P<0.01),运动前推拿24 h和72 h组骨骼肌Ca^(2+)-ATPase浓度较同时段运动后推拿组减少(P<0.05).结论:推拿能有效预防大运动量和长时间运动对肌肉的损伤,可能与推拿提高骨骼肌Ca^(2+)-ATPase活性,增强线粒体对Ca^(2+)的转运有关.

关 键 词：TUINA MASSAGE Manual Therapies Creatine Kinase MYALGIA FATIGUE MITOCHONDRIA RATS 

分 类 号：R2-03[医药卫生—中医学]