离子液体液-液微萃取鸡肉四环素HPLC法的检测  

Determination of Four Tetracyclines in Chicken by Ionic Liquid Liquid-Liquid Micro Extraction Combined With High-Performance Liquid Chromatography

作　　者：林志銮[1] 张传海[1] 马春华 张静 詹宝珍 LIN Zhiluan;ZHANG Chuanghai;MA Chunhua;ZHANG Jin;ZHAN Baozhen(Key Laboratory of Green Technology for Ecological Industry of Fujian Province,Wuyi University,Wuyishan,Fujian 354300;School of Tea and Food,Wuyi University,Wuyishan,Fujian 354300;School of Food Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002)

机构地区：[1]武夷学院福建省生态产业绿色技术重点实验室,福建武夷山354300 [2]武夷学院茶与食品学院,福建武夷山354300 [3]福建农林大学食品科学学院,福建福州350002

出　　处：《武夷学院学报》2022年第12期14-18,共5页Journal of Wuyi University

基　　金：福建省科技厅闽北特色中药材栽培关键技术在产业扶贫中的应用示范与推广大项目(2018L3018);南平市科技局闽北区域多花黄精种质资源保护与开发利用项目(N2020Z013)。

摘　　要：采用超声波辅助离子液体液-液微萃取提取法从鸡肉中提取土霉素(OTC)、四环素(TC)、金霉素(CTC)、多西环素(DCC)等四环素类抗生素,并用高效液相色谱法(HPLC)测定鸡肉中4种四环素的含量。结果表明:以浓度3%,萃取体积450μL[OMIN][PF6]为提取剂,720μL丙酮为分散剂,超声提取时间为15 min。在最优的富集条件下,鸡肉中未检出DCC、CTC、TC和OTC;采用高效液相色谱法,在12 min内分别洗脱DCC、CTC、TC和OTC,检出限分别为0.50、0.10、0.10和0.10μg/mL,DCC的线性范围为0.50~50μg/mL,r≥0.9952。可见用高效液相色谱法能满足快速检测鸡肉中的DCC、CTC、TC和OTC。Four tetracyclines(OTC,TC,CTC,DCC)were extracted from chicken meat by ultrasonic-assisted extraction and dispersive Ionic liquid-assisted micro extraction,and the contents of four tetracyclines in chicken meat were determined by HPLC.The results showed that 1-butyl-3-methylimidazolium was extracted with 3.00%concentration and 450μL of[OMIn][PF6],720μL of acetone was used as dispersant and the extraction time was 15 min.Under these conditions,no DCC,CTC,TC and OTC were detected in chicken meat.DCC,CTC,TC and OTC were all eluted within 12 min by HPLC.The detection limits of DCC,CTC,TC and OTC were 0.50,0.10,0.10,0.10μg/mL respectively.The linear range of DCC was from 0.50 to 50μg/mL,R≥0.9952.The results showed that the DCC,CTC,C and OTC in chicken meat could be detected rapidly by HPLC.

关 键 词：四环素 鸡肉 离子液体 高效液相色谱法 

分 类 号：O657.63[理学—分析化学] TS251.55[理学—化学]