口腔白斑抑癌基因DAPK和TIG1高甲基化表达研究  

Hypermethylation of DAPK and TIG1 in Oral Leukoplakia

作　　者：柳杨 周曾同[2] 龚中坚 吴祥冰 於俊 LIU Yang;ZHOU Zeng-tong;GONG Zhong-jian;WU Xiang-bing;YU Jun(Department of Stomatology,The Affiliated Wuxi People's Hospital of Nanjing Medical University,Wuxi,Jiangsu,214023,China;Department of Oral Mucosal Diseases,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China)

机构地区：[1]南京医科大学附属无锡人民医院口腔科,江苏无锡214023 [2]上海交通大学医学院附属第九人民医院口腔黏膜病科,上海200011

出　　处：《现代生物医学进展》2022年第23期4506-4510,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81502347)。

摘　　要：目的:探讨抑癌基因DAPK、TIG1高甲基化在口腔白斑中表达状态及其对口腔癌发生发展中的作用。方法:取77例口腔白斑、32例口腔鳞癌、32份正常口腔黏膜组织,用实时定量甲基化特异性PCR技术检测组织中DAPK、TIG1高甲基化表达并进行统计学分析。结果:DAPK在口腔鳞癌组织中高甲基化表达率为46.9%,表达量为(0.0728±0.1617),明显高于其在口腔白斑组织(19.5%,0.0070±0.0172)和口腔正常组织(18.8%,0.0021±0.0050)中的表达,差异有统计学意义(P<0.05)。DAPK高甲基化表达与口腔白斑组织上皮异常增生程度相关,上皮增生高风险组相对于低风险组DAPK高甲基化表达风险增加(OR,1.013;95%CI,1.004-1.023;P=0.004)。TIG1高甲基化在正常组织中未表达,在口腔鳞癌组织和口腔白斑组织表达为(28.1%,0.0174±0.0440)和(27.3%,0.0035±0.0076),与正常组织相比具有统计学意义(P<0.05)。结论:抑癌基因DAPK、TIG1高甲基化有望成为口腔黏膜癌变早期标志物。Objective:To detect promoter hypermethylation status of tumor suppressor genes of DAPK and TIG1 in oral leukoplakia(OLK)and explore its potential roles in oral carcinogenesis.Methods:DAPK and TIG1 hypermethylation were evaluated in 77OLK,32 oral squamous cell carcinomas(OSCC)and 40 normal tissues by quantitative methylation-specific PCR(QMSP).Results:It was found that DAPK were hypermethylated in OSCC.The frequency(46.9%)and the quantity(0.0728±0.1617)of DAPK hypermethylation in OSCC was significant higher than those in OLK and normal tissues(OLK:19.5%,0.0070±0.0172;nornal tissues:18.8%,0.0021±0.0050,P<0.05).Furthermore,DAPK hypermethylation was significantly correlated with epithelial dysplasia.Compared with the low malignant potential group of OLK patients(LMP),the high malignant potential group(HMP)was more likely to express increased gene hypermethylation of DAPK(OR,1.013;95%CI:1.004-1.023;P=0.004).No hypermethylation of TIG1 was found in normal tissues.The frequency(OSCC:28.1%;OLK:27.3%)and the quantity(OSCC:0.0174±0.0440;OLK:0.0035±0.0076)of TIG1 hypermethylation were both higher than those in normal tissues(P<0.05).Conclusions:Our data suggested that DAPK and TIG1 hypermethylations were associated with oral precancer and may be useful as early detection markers for oral cancer.

关 键 词：口腔白斑 口腔癌前病变 高甲基化 DAPK TIG1 

分 类 号：R781.5[医药卫生—口腔医学] R739.85[医药卫生—临床医学]