Microbial chassis design and engineering for production of amino acids used in food industry  

作　　者：Jianli Wang Wenjian Ma Jingwen Zhou Xiaoyuan Wang 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,1800 Lihu Avenue,Wuxi 214000,China [2]International Joint Laboratory on Food Safety,Jiangnan University,Wuxi 214122,China [3]Science Center for Future Foods,Jiangnan University,Wuxi 214122,China [4]Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Systems Microbiology and Biomanufacturing》2023年第1期28-48,共21页系统微生物学与生物制造（英文）

基　　金：supported by the National Natural Science Foundation of China(32100023);the Provincial Natural Science Foundation of Jiangsu Province(BK20210466);the National Natural Science Foundation of China(32000020);the Provincial Natural Science Foundation of Jiangsu Province(BK20200615);and the Youth Fund for Basic Research Program of Jiangnan University(JUSRP122009).

摘　　要：Rational microbial chassis design and engineering for improving production of amino acids have attracted a considerable attention.l-glutamate,l-lysine,l-threonine and l-tryptophan are the main amino acids demanded in the food industry.Systems metabolic engineering and synthetic biology engineering generally are believed as the comprehensive engineering approaches to obtain rationally designed strains and construct high-performance platforms for amino acids.The strate-gies focus on microbial chassis characterization optimization,precise metabolic engineering such as promoter engineer-ing,modular pathway engineering,transporter engineering,and dynamic switch systems application,and global genome streamline engineering to reduce cell burden.In this review,we summarized the efficient engineering strategies to optimize Corynebacterium glutamicum and Escherichia coli cell factories for improving the production of l-glutamate,l-lysine,l-threonine,and l-tryptophan.

关 键 词：Microbial chassis Amino acids Food industries Synthetic biology Metabolic engineering Escherichia coli Corynebacterium glutamicum L-GLUTAMATE L-LYSINE L-THREONINE L-TRYPTOPHAN 

分 类 号：TS201.3[轻工技术与工程—食品科学]