CRISPR/Cas9技术在昆虫基因功能研究中的应用及优化  

An application and optimization of CRISPR/Cas9 system in functional analysis of insect genes:A review and prospect

作　　者：刘露露 林哲[2,3] 邹振 LIU Lulu;LIN Zhe;ZOU Zhen(Institute of Life Science and Green Development,School of Life Science,Hebei University,Baoding,Hebei 071002;State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101;CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室,北京100101 [3]中国科学院大学生物互作卓越创新中心,北京100049

出　　处：《热带生物学报》2023年第1期50-59,共10页Journal of Tropical Biology

基　　金：国家自然科学基金(31872298)。

摘　　要：主要概述了CRISPR/Cas9技术应用于涉及鞘翅目、鳞翅目、膜翅目、半翅目、直翅目和双翅目的昆虫在色素沉着、抗性、嗅觉、交配和性别发生等方面的研究进展,包括基因敲除和基因敲入技术的应用,同时总结了目前CRISPR/Cas9系统的优化方案,最后对改良CRISPR/Cas9系统在昆虫基因组学研究和农业害虫防控的应用前景进行了展望。利用基因编辑技术对昆虫基因功能进行探究是除基因过表达、RNA干扰等方法外的一种有效策略。CRISPR/Cas9技术是近年来出现并迅速发展的第三代基因编辑工具,以操作简便、成本低和编辑效率高等优点而被广泛应用于生物科学中的各个分支。In addition to gene overexpression and RNA interference, it is also an effective strategy to use gene editing technology to explore the function of insect genes. CRISPR/Cas9 technology is a third-generation gene editing tool that has developed rapidly in recent years. It is widely used in diverse areas of life science research due to its simple operation, low material cost, and high editing efficiency. A review was made of the application of CRISPR/Cas9 in the pigmentation, insecticide resistance, olfactory transduction, mating, and sex determination of Coleoptera, Lepidoptera, Hymenoptera, Hemiptera, Orthoptera, and Diptera insects, including gene knockout and gene knockin. And recent optimization strategies for the CRISPR/Cas9 system were summarized, based on which a prospect for application of the optimized CRISPR/Cas9 system in insect functional genomics study and agricultural pest management was made.

关 键 词：基因编辑技术 CRISPR/Cas9 基因敲除 基因敲入 

分 类 号：Q78[生物学—分子生物学]