Metabolomic and transcriptomic study to understand changes in metabolic and immune responses in steers under heat stress  

作　　者：Jun Sik Eom Da Som Park Sang Jin Lee Bon-Hee Gu Shin Ja Lee Sang-Suk Lee Seon-Ho Kim Byeong-Woo Kim Sung Sill Lee Myunghoo Kim 

机构地区：[1]Institute of Agriculture and Life Science,Gyeongsang National University,Jinju,52828,Republic of Korea [2]Department of Animal Science,College of Natural Resources&Life Science,Pusan National University,Miryang,50463,Republic of Korea [3]Life and Industry Convergence Research Institute,Pusan National University,Miryang,50463,Republic of Korea [4]University-Centered Labs,Gyeongsang National University,Jinju,52828,Republic of Korea [5]Ruminant Nutrition and Anaerobe Laboratory,Department of Animal Science and Technology,Sunchon National University,Suncheon,57922,Republic of Korea [6]Division of Applied Life Science(BK21),Gyeongsang National University,Jinju,52828,Republic of Korea

出　　处：《Animal Nutrition》2022年第4期87-101,共15页动物营养（英文版）

基　　金：the“Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ015039)”;Rural Development Administration,Korea。

摘　　要：Heat stress(HS)damages livestock by adversely affecting physiological and immunological functions.However,fundamental understanding of the metabolic and immunological mechanisms in animals under HS remains elusive,particularly in steers.To understand the changes on metabolic and immune responses in steers under HS condition,we performed RNA-sequencing and proton nuclear magnetic resonance spectroscopy-based metabolomics on HS-free(temperature humidity index[THI]value:64.92±0.56)and HS-exposed(THI value:79.13±0.56)Jersey steer(n=8,body weight:559.67±32.72 kg).This study clarifies the metabolic changes in 3 biofluids(rumen fluid,serum,and urine)and the immune responses observed in the peripheral blood mononuclear cells of HS-exposed steers.This integrated approach allowed the discovery of HS-sensitive metabolic and immunological pathways.The metabolomic analysis indicated that HS-exposed steers showed potential HS biomarkers such as isocitrate,formate,creatine,and riboflavin(P<0.05).Among them,there were several integrative metabolic pathways between rumen fluid and serum.Furthermore,HS altered mRNA expression and immune-related signaling pathways.A meta-analysis revealed that HS decreased riboflavin metabolism and the expression of glyoxylate and dicarboxylate metabolism-related genes.Moreover,metabolic pathways,such as the hypoxia-inducible factor-1 signaling pathway,were downregulated in immune cells by HS(P<0.05).These findings,along with the datasets of pathways and phenotypic differences as potential biomarkers in steers,can support more in-depth research to elucidate the interrelated metabolic and immunological pathways.This would help suggest new strategies to ameliorate the effects of HS,including disease susceptibility and metabolic disorders,in Jersey steers.

关 键 词：Heat stress Jersey steer METABOLOME Transcriptome Immunity Metabolism 

分 类 号：S823[农业科学—畜牧学]