A reference-grade genome assembly for Gossypium bickii and insights into its genome evolution and formation of pigment glands and gossypol  

作　　者：Kuang Sheng Yue Sun Meng Liu Yuefen Cao Yifei Han Cheng Li Uzair Muhammad Muhammad Khan Daud Wanru Wang Huazu Li Samrana Samrana Yixuan Hui Shuijin Zhu Jinhong Chen Tianlun Zhao 

机构地区：[1]Institute of Crop Science,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,China [2]Novogene Bioinformatics Institute,Beijing,China [3]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,China [4]Department of Biotechnology and Genetic Engineering,Kohat University of Science and Technology,Kohat,Pakistan [5]Hainan Institute,Zhejiang University,Sanya,China

出　　处：《Plant Communications》2023年第1期13-27,共15页植物通讯（英文）

基　　金：supported by the National Key Technology R&D Program of China(2016YFD0101404);the Chinese Agricultural Research System(CARS-18-25);the Jiangsu Collaborative Innovation Center for Modern Crop Production.

摘　　要：The pigment gland is a morphological characteristic of Gossypium and its related genera.Gossypium bickii(G_(1))is characterized by delayed pigment gland morphogenesis in the cotyledons.In this study,a referencegrade genome of G_(1) was generated,and comparative genomics analysis showed that G_(1) was closest to Gossypium australe(G_(2)),followed by A-and D-genome species.Two large fragment translocations in chromosomes 5 and 13 were detected between the G genome and other Gossypiumgenomes and were unique to the G_(1) and G_(2) genomes.Compared with the G_(2) genome,two large fragment inversions in chromosomes 12 and 13 were detected in G_(1).According to the phylogeny,divergence time,and similarity analysis of nuclear andchloroplast genomes,G_(1)was formedby hybridization between Gossypium sturtianum(C_(1))and a common ancestor of G_(2) and Gossypiumnelsonii(G_(3)).The coordinated expression patterns of pigment gland formation(GoPGF)and gossypol biosynthesis genes in G_(1) were verified to be consistent with its phenotype,and nine genes thatwere related to theprocessof pigmentgland formationwere identified.Anovel gene,GbiCYP76B6,regulated by GoPGF,was found to affect gossypol biosynthesis.These findings offer insights into the origin and evolution of G_(1) and its mechanism of pigment gland formation and gossypol biosynthesis.

关 键 词：Gossypium bickii GENOME evolution GOSSYPOL pigment glands 

分 类 号：Q94[生物学—植物学]