LncRNA MEG3在胶质瘤组织中的表达变化及其相关功能  

Expression changes of LncRNA MEG3 in glioma tissues and its related functions

作　　者：张珂珂 张振 郭庚寅 陈金言 何东 ZHANG Keke;ZHANG Zhen;GUO Gengyin;CHEN Jinyan;HE dong(Medical Department,Shandong Provincial Hospital of Shandong First Medical University,Jinan 250012,China;不详)

机构地区：[1]山东第一医科大学附属省立医院医务部,济南250012 [2]山东第一医科大学附属省立医院神经外科

出　　处：《山东医药》2023年第4期11-16,共6页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划项目(202004041407)。

摘　　要：目的基于生物信息学分析长链非编码RNA(LncRNA)母系表达基因3(MEG3)在胶质瘤组织中的表达变化及其相关功能。方法基于人类蛋白质图谱(HPA)数据集分析MEG3在不同组织中的表达,根据癌症和肿瘤基因图谱(TCGA)数据集应用R软件分析其在泛癌中的表达,进一步使用泛胶质瘤数据集(TCGA-GBMLGG)中的临床病理特征资料分析MEG3在胶质瘤与正常脑组织的表达关系,同时分析不同WHO分级以及胶质瘤重要分子病理标志(IDH突变与1p/19q共缺失)与MEG3表达的相关性;并应用临床预后数据绘制各MEG3亚组患者的生存曲线,用基因型-组织表达(GTEx)数据库匹配TCGA-GBMLGG数据集,绘制受试者工作特征曲线评估其诊断效能。应用Linkedomics数据库中的TCGA-GBMLGG数据分析MEG3共表达基因的功能富集情况,应用R软件DESeq2软件包分析以MEG3作为亚组的不同亚组间差异表达基因,用Metascape数据库分析差异基因的表达,用基因集富集(GESA)分析全部差异表达基因富集的功能通路,单样本基因集富集(ssGESA)与肿瘤免疫估算数据库(TIMER)分析MEG3表达与胶质瘤免疫浸润状态的关系。结果MEG3在胶质瘤组织中低表达,与1p/19q共缺少相关;MEG3可区分正常脑组织与胶质瘤组织。MEG3相关表达基因的功能富集结果显示,其与神经系统与免疫功能密切相关;MEG3的差异基因功能富集结果显示其与干扰素反应相关,主要影响巨噬细胞、细胞毒性细胞的免疫浸润状态,并且不同MEG3分组的临床样本中多种免疫细胞富集分数具有显著差异,提示MEG3低表达可能与胶质瘤的免疫抑制微环境相关。结论MEG3在胶质瘤组织中低表达,可作为胶质瘤的诊断标志物与预后标志物,有望成为胶质瘤免疫治疗和相关研究的重要靶点。Objective To analyze the expression characteristics of(LncRNA)MEG3 in the glioma tissues and its related functions using bioinformatics methods.Methods We analyzed the expression pattern of MEG3 in different tissues based on HPA dataset,analyzed its expression in pan-cancer using TCGA dataset applying R software,and further analyzed the expression relationship of MEG3 in glioma tissues and normal brain tissues by using clinicopathologic features from TCGA-GBMLGG.Meanwhile,we analyzed the correlations between MEG3 expression and different tumors with different WHO classifications and important molecular pathological markers(IDH mutation with 1p/19q co deletion).Diagnostic efficacy was assessed by applying clinical prognostic data to plot survival curves for different MEG3 subgroups and receiver operating characteristic(ROC)curves using the GTEx database matching the TCGA-GBMLGG dataset.The TCGA-GBMLGG data from the Linkedomics database was applied to demonstrate the functional enrichment of MEG3 co-expressed genes.The R software DESeq2 package was applied to analyze differential gene expression between different subgroups using MEG3 as a subgroup,and the Metascape database was used to analyze differential gene expression.Finally,GESA was applied to analyze the functional pathways of all differential gene enrichment,and ssGESA was used with TIMIER database to analyze the correlation between the status of MEG3 expression and glioma immune infiltration.Results MEG3 was low expressed in glioma tissues and related to the clinicopathological features of 1p/19q.MEG3 could distinguish the normal brain tissues from glioma;functional enrichment of MEG3-related expressed genes showed that it was closely associated with neurological and immune functions;functional enrichment of differential genes of MEG3 showed that it was associated with interferon response and mainly affected macrophages,and immune infiltration of cytotoxic cells.The differential functional enrichment of MEG3 showed that the low expression of MEG3 was relat

关 键 词：胶质瘤 长链非编码RNA 母体表达基因3 生物信息学 免疫浸润 

分 类 号：R739.4[医药卫生—肿瘤]