南京腹泻患儿轮状病毒感染与受体组织血型抗原相关性研究  

Study on the correlation between rotavirus infection and Histo-Blood Group Antigens in children with diarrhea in Nanjing

作　　者：庄著伦[1] 闫坤龙[1] 金玉[1] 刘志峰[1] 程卫霞[1] Zhuang Zhulun;Yan Kunlong;Jin Yu;Liu Zhifeng;Cheng Weixia(Children′s Hospital of Nanjing Medical University,Nanjing 210008,China)

机构地区：[1]南京医科大学附属儿童医院,南京210008

出　　处：《中华实验和临床病毒学杂志》2022年第6期691-695,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：南京市医学科技发展资金(QRX17165)。

摘　　要：目的了解南京地区5岁以下腹泻患儿轮状病毒感染及流行基因型与受体人组织血型抗原易感性。方法收集295名急性腹泻儿童和150名健康儿童粪便和相应的唾液样本;应用抗原检测方法对粪便样本进行轮状病毒检测,利用RT-PCR方法对轮状病毒阳性样本基因分型;利用抗组织血型抗原单克隆抗体ELISA方法对唾液样本进行人组织血型抗原表型测定;对轮状病毒感染与组织血型抗原易感性进行统计分析。结果病例组295例标本中139例(47.12%)为轮状病毒阳性,其中G9[P8]型为最常见的轮状病毒基因型(84.17%,117/139)。轮状病毒感染患儿出现发热(χ^(2)=34.81,P<0.001)、呕吐(χ^(2)=25.01,P<0.001)和合并呼吸道症状比例(χ^(2)=4.73,P=0.03),均高于非轮状病毒感染组,差异有统计学意义。在ABO血型系统中,AB型儿童更易发生腹泻(95%CI:1.029~2.622;P=0.036),B型儿童感染轮状病毒的风险更高(OR=1.783,95%CI:1.027~3.095,P=0.039);在分泌型系统中,分泌型儿童更易发生腹泻和感染轮状病毒,G9[P8]基因型感染与分泌型表型有关(OR=2.854,95%CI:1.641~4.962),非分泌型儿童不易感染轮状病毒和G9[P8]基因型轮状病毒(χ^(2)=5.723,P=0.017)。结论南京地区5岁以下腹泻儿童轮状病毒感染以G9[P8]基因型为主,分泌型表型个体更易感染G9[P8],为预防和控制该地区轮状病毒腹泻提供科学依据。Objective To investigate the prevalence of rotavirus infection and susceptibility to Histo-Blood Group Antigens(HBGAs)in children with diarrhea under 5 years of age in Nanjing.Methods Stool and corresponding saliva samples were collected from 295 children with acute diarrhea and 150 healthy children.Rotaviruses were detected in stool samples by antigen detection method,and rotavirus positive samples were genotyped by RT-PCR method.HBGA phenotype of saliva samples was determined by anti-tissue blood group monoclonal antibody ELISA method.Rotavirus infection and HBGA susceptibility were analyzed by statistical analysis.Results In the case group,139(47.12%)of 295 samples were rotavirus positive,with G9[P8]genotype being the most common genotype(84.17%,117/139).The proportion of fever(χ^(2)=34.81,P<0.001),vomiting(χ^(2)=25.01,P<0.001)and respiratory symptoms(χ^(2)=4.73,P=0.03)in rotavirus infected children,which were higher than those in non-rotavirus infected group,and the difference was statistically significant.In the ABO blood group system,type AB children were more likely to have diarrhea(95%CI:1.029~2.622;P=0.036),and type B children had a higher risk of rotavirus infection(OR=1.783,95%CI:1.027~3.095,P=0.039).In the secretory system antigens,secretory children were more prone to diarrhea and rotavirus infection,G9[P8]genotype infection was related to secretory phenotype(OR=2.854,95%CI:1.641~4.962),and non-secretory children(χ^(2)=5.723,P=0.017)were less susceptible to rotavirus and G9[P8]genotype rotavirus infection.Conclusions G9[P8]genotype was the main rotavirus infection in diarrhea children under 5 years of age in Nanjing,and individuals with secretory phenotype were more likely to be infected with G9[P8],which provided scientific basis for preventing and controlling rotavirus diarrhea in this area.

关 键 词：腹泻 轮状病毒 组织血型抗原 病例对照研究 

分 类 号：R725.7[医药卫生—儿科]