Study on the effects of alternating capacitive electric fields with different frequencies on promoting wound healing  

作　　者：Yunxue Jia Junwei Xu Qiusheng Shi Lisha Zheng Meili Liu Ming Wang Ping Li Yubo Fan 

机构地区：[1]Key Laboratory of Biomechanics and Mechanobiology of Ministry of Education,Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,Beijing,100083,China [2]Beijing Key Laboratory of Bioelectromagnetism,Institute of Electrical Engineering,Chinese Academy of Sciences,Beijing,100190,China [3]School of Medical Science and Engineering,Beihang University,Beijing,100083,China

出　　处：《Medicine in Novel Technology and Devices》2022年第4期42-50,共9页医学中新技术与新装备（英文）

基　　金：National Natural Science Foundation of China(NSFC)Research Grants(61871014,52072015,31971238,52071008,U20A20390,11827803);it as also supported by National Key R&D Program of China(2017YFC0108505,2017YFC0108500);Beijing Natural Science Foundation(7191006).

摘　　要：Some researches to facilitate wound healing by using electrical stimulation are based on electric current stimulation,which may cause secondary damage and the imbalance of the microenvironment in vivo.In this study,alternating capacitive electric field(ACEF)was applied via a self-designed system so as to avoid direct contact with cells and to maintain stable microenvironment for cell growth.The influences of 58 mV/mm ACEFs with various frequencies of 10,60 and 110 Hz on epidermal cells,fibroblasts and macrophages which involve in wound healing were comprehensively explored.The results suggested that ACEFs of 10,60 and 110 Hz all significantly promoted the proliferation of human dermal fibroblasts(HDFs)and human epidermal keratinocyte cell line(HaCaT)cells,and 60 Hz ACEF furtherly accelerated the migration of these two kinds of cells.Moreover,ACEFs of all different studied frequencies facilitated M2-type polarization of macrophages,and YAP/TAZ expression of macrophages were enhanced under the stimulations of 10 and 60 Hz ACEFs.The enhancements in cell activity,migration rate and M2-type polarizability indicated that 58 mV/mm ACEFs especially at 60 Hz possessing potentially affirmative applications for wound healing without the risks of secondary damage and microenvironment imbalance.

关 键 词：Alternating capacitive electric field Frequency Human dermal fibroblasts Human epidermal keratinocyte cell line MACROPHAGES Wound healing 

分 类 号：R641[医药卫生—外科学]