CD4 cell activation with the CD8 marker and metallothionein expression in the gills of cadmium-exposed mosquito fish(Gambusia affinis Baird and Girard 1853)juveniles  

作　　者：Moh Awaludin Adam Agoes Soegianto Carolyn Melissa Payus Ach Khumaidi Ramli Ramli Ernawati Ernawati Irawati Mei Widiastuti Era Insivitawati Yenny Risjani 

机构地区：[1]Faculty of Science and Technology,University of Ibrahimy,Situbondo,Indonesia [2]Research Center for Marine and Land Bioindustry,National Research and Innovation Agency,Lombok,NTB,Indonesia [3]Department of Biology,Faculty of Science and Technology,Universitas Airlangga,Surabaya,Indonesia [4]Faculty of Science and Natural Resources,Universiti Malaysia Sabah,Kota Kinabalu,Sabah,Malaysia [5]Aquatic Product Technology Department,Faculty of Agriculture,University of Yudharta,Pasuruan,East Java,Indonesia [6]Aquaculture Department,Faculty of Animal Husbandry and Fisheries,University of Tadulako,Palu,Central Sulawesi,Indonesia [7]Politeknik Kementerian Kelautan dan Perikanan,Sidoarjo,Jawa Timur,Indonesia [8]Department of Aquatic Resources Management,Faculty of Fisheries and Marine Sciences,Brawijaya University,Malang,Indonesia

出　　处：《Emerging Contaminants》2022年第1期280-287,共8页新兴污染物（英文）

基　　金：Ministry Education and Culture,Directorate of Research and Technology for Research and Innovation,grant number 028/AMD-SP2H/LT-MULTI-PDPK/LL7/2021.

摘　　要：This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis gills.Four treatment groups were used,with one control and treatments A,B,C,and D at dosages of 0.03 mg/L,0.023 mg/L,0.015 mg/L,and 0.008 mg/L,respectively.The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control(0.33%).The value in A,namely 0.54%,was the highest,followed by B,C,and D of 0.46%,0.44%,and 0.42%,respectively.The number of macrophages increased as the Cd level of the medium increased,as did the activity of pinocytosis.Furthermore,the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills.Similarly,the MT intensity increased at Cd-exposed gills as compared to the control.As the Cd level in the gills increased,the MT levels ascended significantly.

关 键 词：CADMIUM CD4 cell Fish health Immunity METALLOTHIONEIN Water pollution 

分 类 号：X52[环境科学与工程—环境工程]