Molecular identification,expression pattern,and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus  

作　　者：Shuang JIAO Xungang TAN Feng YOU Shujing ZHANG Qiuxiang PANG 

机构地区：[1]Anti-Aging&Regenerative Medicine Research Institution,School of Life Sciences and Medicine,Shandong University of Technology,Zibo 255000,China [2]CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China [3]Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China

出　　处：《Journal of Oceanology and Limnology》2023年第1期280-289,共10页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(No.31972774);the National Natural Science Foundation of China(No.31672636);the Key Research and Development Program of Shandong Province,China(No.2019GHY112007)。

摘　　要：Insulin-like growth factors(IGFs)are key regulators of development and growth.Here,we characterized the igf2 gene from olive flounder(Paralichthys olivaceus)and determined its temporal and spatial expression.We set up an in-vitro protein expression system in eukaryotic human embryonic kidney(HEK293 T)cells and explored its effects on cell proliferation.The flounder igf2 cDNA contained a 648-bp open reading frame(ORF)encoding a protein of 215 amino acids(aa),which spanned the complete signal peptide(47 aa),mature peptide(70 aa),and E domain(98 aa).In adult flounder,igf2 mRNA was detected in all selected tissues.In early development,igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos.In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region,floor plate,hypochord,otic vesicle,and pectoral fin during embryogenesis.Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells.In addition,the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma(HeLa)and HEK293T cells.These results provide new information about the structural and functional conservation,expression patterns,and biological activity of the igf2 in teleosts.

关 键 词：AQUACULTURE FLATFISH IGF2 PROLIFERATION 

分 类 号：S917.4[农业科学—水产科学]