新型荧光比率传感器的构建及对抗坏血酸检测  

Construction of a novel fluorescence ratiometric sensor and its detection for ascorbic acid

作　　者：翟红[1] 孙豆 张雪然 高萌萌 ZHAI Hong;SUN Dou;ZHANG Xueran;GAO Mengmeng(College of Chemistry and Chemical Engineering,Shanxi Datong University,Datong 037009,China)

机构地区：[1]山西大同大学化学与化工学院,大同037009

出　　处：《分析试验室》2022年第11期1269-1273,共5页Chinese Journal of Analysis Laboratory

基　　金：山西省高等学校科技创新项目(2019L0759);山西省高等学校大学生创新创业训练计划项目(2021585);山西大同大学大学生创新创业训练计划项目(XDC2020119)资助。

摘　　要：以邻苯二胺(OPD)为潜在荧光响应分子,罗丹明B掺杂纳米硅球(RBITC@SiO_(2))的荧光强度为参比信号,构建双发射荧光比率传感器,并用于抗坏血酸(AA)的测定。AA与OPD在碱性条件下可生成发蓝色荧光的3-二羟乙基呋喃[3,4-b]喹喔啉-1-酮(OA)。结果表明,OA与RBITC@SiO_(2)的荧光比率(F_(421)/F_(578))与AA浓度在0~300μmol/L内呈线性关系,检出限为1.42μmol/L。通过计算F_(421)/F_(578),可实现对AA的准确测定。A dual-emission fluorescence ratio sensor was constructed with o-phenylendiamine(OPD)as the potential fluorescence response molecule and the fluorescence intensity of silica nanoparticle doped with rhodamine B(RBITC@SiO_(2))as the reference signal,and was used for the determination of ascorbic acid(AA).AA and OPD could produce 3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one(OA)with blue fluorescence under alkaline condition.The results showed that the fluorescence ratio(F_(421)/F_(578))of OA and RBITC@SiOhad a linear relationship with AA concentration in the range of 0-300μmol/L,and the detection limit was 1.42μmol/L.By calculating F_(421)/F_(578),accurate determination of AA could be realized.

关 键 词：掺杂罗丹明B纳米硅球 比率传感器 邻苯二胺 抗坏血酸 

分 类 号：O657.3[理学—分析化学]