基于二氧化锰纳米片层比色法检测抗氧化物质  

Colorimetric detection of antioxidants based on MnO_(2) nanosheets

作　　者：赵灵芝[1] 赵柳 张小清[1] 王龙杰 陈楠 ZHAO Lingzhi;ZHAO Liu;ZHANG Xiaoqing;WANG Longjie;CHEN Nan(Department of Pharmacy,Xi'an Medical College,Xi'an 710021,China;Shanxi Agricultural Inspection and Testing Center,Xi'an 710003,China)

机构地区：[1]西安医学院药学院,西安710021 [2]陕西省农业检验检测中心,西安710003

出　　处：《分析试验室》2022年第11期1302-1306,共5页Chinese Journal of Analysis Laboratory

基　　金：西安医学院杰出人才青年计划配套项目(2021PT08);西安医学院药学省级重点学科建设项目(2016YXXK15);陕西省科技厅项目(2018JM2027,2019JQ-890);陕西省教育厅项目(18JK0655);2020年陕西省大学生创新项目(S202011840031,121520031)资助。

摘　　要：基于MnO_(2)纳米片层和四甲基联苯胺(TMB)显色反应,建立了检测多种抗氧化物质的比色法。在最佳条件下,吸光度与各抗氧化物质的浓度呈良好的线性关系。考察了该显色体系在纸芯片上检测的可行性,所构建的纸基比色体系可用于临床和现场检测抗氧化物质。A colorimetric method for the detection of various antioxidants was developed based on the chromogenic reaction of manganese dioxide nanosheets with tetramethylbenzidine(TMB).Under the optimal conditions,the absorbance intensity exhibited favourable quantitative relationships with the concentrations of antioxidants.The feasibility of the chromogenic system exploited on paper chip detection was investigated.The paper-based colorimetric system showed potential prospect in clinical application and field detection of antioxidants.

关 键 词：MnO_(2)纳米片层 3 3’ 5 5’-四甲基联苯胺(TMB) 抗氧化物质 纸基比色法 

分 类 号：O657.3[理学—分析化学]