不同基团修饰聚苯乙烯微塑料对大型溞的影响及其在肠道中的积累  

Effects of polystyrene microplastics modified by different groups on Daphnia magna and their intestinal accumulation

作　　者：王超 李佳洁[1] 方智新 邓洁薇 栾天罡 WANG Chao;LI Jiajie;FANG Zhixin;DENG Jiewei;LUAN Tiangang(School of Biomedical and Pharmaceutical Sciences,Guangdong University of Technology,Guangzhou 510006,China;Guangdong Second Provincial General Hospital,Guangzhou 510399,China;School of Life Sciences,Sun Yat-Sen University,Guangzhou 510275,China)

机构地区：[1]广东工业大学生物医药学院,广州510006 [2]广东省第二人民医院,广州510399 [3]中山大学生命科学学院,广州510275

出　　处：《分析试验室》2022年第12期1556-1561,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(22127810,22076027);广东省自然科学基金(2022A1515011980,2021A1515010196)项目资助。

摘　　要：选择3种结构不同的绿色荧光聚苯乙烯微塑料(聚苯乙烯微球(PS)、表面键合羧基聚苯乙烯微球(PS-COOH)和表面键合氨基聚苯乙烯微球(PS-NH2))为研究目标,探讨其对淡水模式生物大型溞的影响及其在肠道中的积累情况。急性毒性实验结果表明,PS,PS-COOH和PS-NH2的72 h半数致死浓度(LC50)分别为12.29,41.36和24.89 mg/L。显微荧光成像结果表明,3种微塑料主要在大型溞的肠道积累,其中低浓度暴露组(50μg/L)在72 h微塑料生物积累达到最大,高浓度组(500μg/L)在24~48 h微塑料生物积累达到最大。将暴露的大型溞转移至不含微塑料的纯培养液中,1 h即可观察到微塑料通过肠道开始排出体外,低浓度组的微塑料在12 h后基本排出,高浓度组的微塑料在36 h基本排出。Three kinds of green fluorescent polystyrene microplastics with different structures(polystyrene microspheres(PS),surface bonded carboxyl polystyrene microspheres(PS-COOH)and surface bonded amino polystyrene microspheres(PS-NH2))were selected as the research objectives.The effects of these three polystyrene microplastics on Daphnia magna a freshwater model organism and their intestinal accumulation were investigated.The results of acute toxicity test showed that the 72 h lethal concentrations of PS,PS-COOH and PSNH2were 12.29,41.36 and 24.89 mg/L,respectively.Microscopic fluorescence imaging results demonstrated that the three microplastics mainly accumulated in the intestine of Daphnia magna.Microplastics reached maximum bioaccumulation at 72 h for the low-concentration exposure group(50μg/L),and reached maximum bioaccumulation at 24-48 h for the high-concentration group(500μg/L).It was observed that the microplastics started to depurate from intestine when the exposed Daphnia magna was transferred to culture medium within1 h.The microplastics in the low-concentration group were almost depurated after 12 h,and that in the high concentration group were almost depurated at 36 h.

关 键 词：微塑料 大型溞 肠道 积累 毒性 

分 类 号：X171.5[环境科学与工程—环境科学]