L-天冬氨酸离子液体联合猪胰脂肪酶催化油酸酯化工艺  

Esterification of oleic acid catalyzed by L-Aspartic acid ionic liquid combined with porcine pancreatic lipase

作　　者：郑礼 汪文伟 王昌梅[1] 王志山 许津萍 尹芳[1] 杨斌[1] 吴凯 赵兴玲[1] 梁承月 柳静[1] 杨红[1] 张无敌[1] ZHENG Li;WANG Wenwei;WANG Changmei;WANG Zhishan;XU Jinping;YIN Fang;YANG Bin;WU Kai;ZHAO Xingling;LIANG Chengyue;LIU Jing;YANG Hong;ZHANG Wudi(School of Energy and Environment Science,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学能源与环境科学学院,昆明650500

出　　处：《中国油脂》2022年第12期126-130,共5页China Oils and Fats

基　　金：云南省万人计划产业技术领军人才项目(20191096);云南省农村能源工程重点实验室基金项目(2017KF03);云南省教育厅科学研究基金项目(218JS146);云南师范大学2020年大学生科研训练基金项目(KX2020140);云南师范大学研究生科研创新基金项目(YJSJJ21-A06)联合资助

摘　　要：为绿色高效制备生物柴油,利用L-天冬氨酸离子液体([Asp]HSO4)联合猪胰脂肪酶催化油酸制备油酸甲酯,采用单因素实验探究了反应时间、醇酸物质的量比、[Asp]HSO4用量、猪胰脂肪酶用量和反应温度对转化率的影响,在此基础上,采用正交实验进行优化,得到[Asp]HSO4联合猪胰脂肪酶催化油酸酯化反应的最优工艺条件为[Asp]HSO4用量6%、醇酸物质的量比5.5∶1、猪胰脂肪酶用量4%、反应时间21 h、反应温度45℃,在此条件下转化率可达91.91%。[Asp]HSO4可降低甲醇和温度对猪胰脂肪酶催化活性的影响,对猪胰脂肪酶催化油酸酯化具有协同效应。In order to prepare biodiesel greenly and efficiently,L-Aspartic acid ionic liquid([Asp]HSO4)combined with porcine pancreatic lipase was used to catalyze the esterification of oleic acid to prepare methyl oleate.The effects of reaction time,molar ratio of methanol to oleic acid,amount of[Asp]HSO4,amount of porcine pancreatic lipase,and the reaction temperature on the conversion rate were investigated by single factor experiment.Then the esterification process conditions were optimized by orthogonal experiment as follows:amount of[Asp]HSO46%,molar ratio of methanol to oleic acid 5.5∶1,amount of porcine pancreatic lipase 4%,reaction time 21 h,and reaction temperature 45℃.The conversion rate could reach 91.91%under the optimal conditions.[Asp]HSO4can reduce the effects of methanol and temperature on the catalytic activity of porcine pancreatic lipase,and has synergistic effect on the esterification of oleic acid catalyzed by porcine pancreatic lipase.

关 键 词：油酸甲酯 天冬氨酸离子液体 脂肪酶 酯化反应 

分 类 号：TQ645[化学工程—精细化工] TK63[动力工程及工程热物理—生物能]