基于MALDI-MS的组织上脂肪酸原位衍生化综合实验教学设计  

Comprehensive experimental design for in-situ derivatization of fatty acids based on MALDI-MS

作　　者：王珊珊 王康[1] WANG Shanshan;WANG Kang(College of Science,Chang’an University,Xi’an,710064,China)

机构地区：[1]长安大学理学院,陕西西安710064

出　　处：《实验技术与管理》2023年第1期138-142,147,共6页Experimental Technology and Management

基　　金：陕西省自然科学基金项目(2021JQ-223)。

摘　　要：针对MALDI-MS在低丰度脂肪酸分析时的难点,将分析化学中的原位衍生化技术应用到脂肪酸MALDI-MS分析中,对小鼠心肌组织、脑组织和甲状腺肿瘤组织中低丰度的脂肪酸进行了原位衍生化,并对衍生化产物采用高分辨质谱和串联质谱技术进行了分析验证。该实验不仅能够使学生熟练掌握大型质谱仪器的实验原理和操作方法,还能够培养学生从生物样本获得、样本前处理到组织上原位衍生化及高分辨质谱数据分析与处理等多方面能力。In view of the difficulties in the analysis of low-abundance fatty acids by MALDI-MS,the in-situ derivatization technology of analytical chemistry was applied to the analysis of fatty acids by MALDI-MS,and the low-abundance fatty acids in mouse cardiac tissue,brain tissue,and human thyroid tumor tissue were in-situ derivatized.The derivatized products were analyzed and verified by high-resolution mass spectrometry and tandem mass spectrometry.This experiment not only enables students to master the experimental principle and operation method of large-scale precision instruments but also cultivates students'abilities to obtain biological samples,sample pre-processing,in-situ derivatization,and the analysis and processing of high-resolution mass spectrometry data.

关 键 词：基质辅助激光解吸离子化 质谱 原位衍生化 脂肪酸 

分 类 号：O657.63[理学—分析化学] G642.0[理学—化学]