大黄鱼trim38基因的克隆及其表达分析  

Study on the Immune Function of trim38 Gene in Larimichthys crocea

作　　者：苟涛 王志勇[1,2] GOU Tao;WANG Zhiyong(Fisheries College,Jimei University,Xiamen 361021,China;Key Laboratory of Healthy Aquaculture for the East China Sea,Ministry of Agriculture and Rural Affairs,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,福建厦门361021 [2]农业农村部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《集美大学学报（自然科学版）》2022年第6期481-488,共8页Journal of Jimei University：Natural Science

基　　金：国家自然科学基金重点支持项目(U1705231);厦门市科技项目(3502Z20193052)。

摘　　要：由变形假单胞菌(Pseudomonas plecoglossicida)引起的大黄鱼(Larimichthys crocea)内脏白点病的死亡率可达50%~80%。对抗病相关基因trim38进行了分子及表达特征等分析。结果显示:该基因开放阅读框(ORF)1623 bp,编码540个氨基酸,预测蛋白分子量为61.58 ku,等电点6.99。qRT-PCR的结果表明,在所有检测的组织中都发现了trim38的转录产物,但在肾脏中的相对表达量最高。变形假单胞菌攻毒刺激后,大黄鱼脾脏中该基因持续表达,在120 h达到峰值;肝脏中3 h显著表达,之后相对表达量降低;肾脏中该基因相对表达量变化不大,在96 h左右达到最大值。上述结果表明trim38基因参与了大黄鱼应对变形假单胞菌感染的抗病免疫过程。Visceral white spot disease caused by Pseudomonas plecoglossicida is one of the main diseases of large yellow croaker cultured.The infection rate reaches more than 50%.In this study,a candidate disease-resistance-related gene trim38 was screened from the above-located major regions,and its molecular and expression characteristics were analyzed.The results showed that the gene open reading frame(ORF)was 1623 bp,encoding 540 amino acids,the predicted protein molecular weight was 61.58 ku,and the isoelectric point was 6.99.The results of qRT-PCR showed that trim38 mRNA transcripts were found in all detected tissues,but the highest relative expression was in the kidney.After challenge with P.plecoglossicida,the gene was continuously expressed in the spleen of large yellow croaker and reached a peak at 120 h;it was significantly expressed in the liver at 3 h and then decreased;the relative expression of the gene in the kidney did not change much and reached the maximum at about 96 h value.These results indicated that trim38 was involved in the immune process of large yellow croaker in response to P.plecoglossicida infection.

关 键 词：大黄鱼 内脏白点病 trim38基因 表达 

分 类 号：S917.4[农业科学—水产科学]