Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines  

作　　者：Hao Chen Faqian Xiong Rilong Xu Xiangyu Chen Haifeng Zhong Yumei Zhang Xinlong Lan Hong Zhang Yuhua Chen Runfang Hu Guoqiang Lin Zhaoxiu Tang 

机构地区：[1]Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fujian Research Station of Crop Gene Resource&Germplasm Enhancement,Ministry of Agriculture and Rural Affairs of People’s Republic of China,Fujian Engineering Research Center for Characteristic Upland Crops Breeding,Fujian Engineering Laboratory of Crop Molecular Breeding,Fuzhou,350013,China [2]Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,530007,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第2期537-557,共21页国际实验植物学杂志（英文）

基　　金：funded by the Basic Scientific Research Special Project for Fujian Provincial Public Research Institutes(2020R10310011,2019R1031-12);the National Natural Science Foundation of China(32001577);the Foreign Cooperation Projects for FAAS(DWHZ2021-20);the Opening Foundation of Fujian Key Laboratory for Vegetable Breeding(FJVRC2020-02);the Free Explore Program for FAAS(ZYTS2019007);the Guangxi Natural Science Foundation Program(2018GXNSFDA281027);the Science and Technology Innovative Team in Fujian Academy of Agricultural Sciences(CXTD2021008-3).

摘　　要：Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.

关 键 词：PEANUT EMS-induced mutant line pod-related traits RE-SEQUENCING pod constriction 

分 类 号：S565.2[农业科学—作物学]