Chickpea C2H2-Type Zinc Finger Protein ZF2 is a Positive Regulator in Drought Response in Arabidopsis  

作　　者：Sushuang Liu Yanmin Liu Chundong Liu Xingwang Yu Hao Ma 

机构地区：[1]Department of Life Sciences and Health,Huzhou College,Huzhou,313000,China [2]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing,210095,China [3]Department of Crop and Soil Sciences,North Carolina State University,Raleigh,27695-7620,USA [4]Institute of Desert in the Arid Areas,College of Grassland and Environment Sciences,Xinjiang Agricultural University,Urumqi,830052,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第2期577-590,共14页国际实验植物学杂志（英文）

基　　金：sponsored by the National Natural Science Foundation of China(31160306 and 30860152);Natural Science Foundation of Zhejiang Province(LQ20C130003);Scientific Research Fund of Zhejiang Provincial Education Department(Y202145972 and Y202248468);Zhejiang Students’Technology and Innovation Program(Xinmiao Program)(2022R485A003).

摘　　要：Drought is a major abiotic stress limiting agricultural crops production worldwide.In our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea.ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain.Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein.In the yeast assay system,the full-length of ZF2 did not show transcriptional activation.Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones.The promoter region of ZF2 contained multiple stress-and hormone-related cis-elements.Overexpression of ZF2 in Arabidopsis significantly improved the root length and fresh weight at seedling stage and enhanced the survival rates and proline content under drought treatment.These results indicated that ZF2 functioned as a positive regulator in drought response.

关 键 词：ZF2 CHICKPEA drought tolerance TRANSGENIC 

分 类 号：Q945.78[生物学—植物学]