牛樟芝的分离鉴定及最适谷物皿培基质的筛选  

Isolation and Identification of Antrodia camphorata and Screening of the OptimalSubstrate of Petri-dish Culture with Grains

作　　者：白雪 王玲芝 余锦欣 章文贤[1] BAI Xue;WANG Ling-zhi;YU Jin-xin;ZHANG Wen-xian(College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117

出　　处：《福建农业科技》2022年第11期10-16,共7页Fujian Agricultural Science and Technology

基　　金：福建省自然科学基金项目(2017J01624)。

摘　　要：牛樟芝是一种中国台湾地区特有的真菌,具有极高的药用和商业价值。皿培式固态培养已经成为牛樟芝主要的人工培养方式之一,筛选最适培养基质成为皿培法的关键。首先从牛樟木培养的牛樟芝子实体中分离得到的菌株BX1119.1,经形态特征、ITS鉴定和系统发育分析确定为台湾牛樟芝种Antrodiacamphorata;接着以麸皮为基本成分,按1∶1(w/w)比例分别加入5种谷物作为碳源,混合得到谷物基质;接种已鉴定菌株后,采用谷物皿培法培养20 d,分别测量接种第5、8、11、14、17、20 d的菌丝生长直径;采用高氯酸-香草醛显色法分别测定不同谷物基质培养的牛樟芝中三萜类化合物含量。试验结果表明最适牛樟芝菌株生长的碳源为黄米粉,最适三萜合成的碳源为莜麦粉。研究结果为牛樟芝人工培养皿培法中固态基质的选择提供了新的解决方案。Antrodia cinnamomea was a unique fungus in Taiwan of China,with extremely high medicinal and commercial value.The solid-state culture of petri dish has become one of the main artificial culture methods for Antrodia cinnamomea.And screening the optimal culture medium was the key to the culture method.Firstly,the strain BX1119.1 was isolated from the fruiting body of Antrodia cinnamomea cultured from the camphor wood,which was identified as Antrodia camphorata through the morphological characteristics,ITS identification and phylogenetic analysis.Then,with the wheat bran as the basic component,five kinds of grains were added as the carbon sources at the ratio of 1∶1(w/w)to obtain the grain matrix by mixing.After the inoculation with the identified strains,the strains were cultured for 20 days by using the grain petri-dish culture method,and the mycelial growth diameters were measured on the 5 th,8 th,11 th,14 th,17 th and 20 th days after the inoculation.The content of triterpenoids in Antrodia camphorata cultured in different grain substrates was determined by using the perchloric acid-vanillin chromogenic method.The results showed that the optimum carbon source for the growth of Antrodia camphorata was yellow rice powder,and the optimum carbon source for the biosynthesis of triterpene was the naked oats flour.The results of this study provided a new solution for the selection of solid matrix in the artificial culture of Antrodia camphorata.

关 键 词：牛樟芝 ITS鉴定 系统发育树 谷物皿培 三萜化合物 

分 类 号：S567.3[农业科学—中草药栽培]