Maternal hyperthyroidism increases the synthesis activity and the osteogenic markers expression of calvarial osteoblasts from offspring in a murine model  

作　　者：FABIANA ROCHA ARAÚJO BRUNO MACHADO BERTASSOLI ISABELLA CRISTINA SOUZA FÉLIX DOUGLAS MARINHO ABREU NATÁLIA MELO OCARINO AMANDA MARIA SENA REIS JUNEO FREITAS SILVA ROGÉRIA SERAKIDES 

机构地区：[1]Núcleo de Células Tronco e Terapia Celular Animal(NCT-TCA),Veterinay School,Universidade Federal de Minas Gerais,Belo Horizonte,Minas Gerais,31270-901,Brasil [2]Universidade de Uberaba(UNIUBE),Uberaba,Minas Gerais,38065-300,Brasil [3]Instituto de Ciências Biológicas,Universidade Federal de Minas Gerais,Belo Horizonte,Minas Gerais,31270-901,Brasil [4]Universidade Estadual de Santa Cruz,Campus Soane Nazare de Andrade,Ilheus,Bahia,Brasil

出　　处：《BIOCELL》2023年第2期423-430,共8页生物细胞（英文）

基　　金：supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq);Fundação de Amparo a Pesquisa de Minas Gerais(Fapemig);Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(Capes).

摘　　要：To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal hyperthyroidism.Twelve adult Wistar rats were divided into two groups,one control and one treated with daily administration of L-thyroxine by an orogastric tube(50μg/animal/day)during pregnancy.Three days after delivery and confirmation of the mothers’hyperthyroidism,the offspring were euthanized for the extraction of osteoblasts from the calvaria.At 7,14,and 21 days,proliferation activity was assessed using MTT assay,while alkaline phosphatase(ALP)activity was assessed by the BCIP/NBT method.At 21 days,the total area of the mineralized matrix stained by von Kossa was evaluated by morphometry.The expression of gene transcripts for Runx2,Bmp2,Fgfr1,collagen type 1(Col1),osteocalcin(Oc),and osteopontin(Op)were evaluated by real-time RT-PCR.Means were compared using the Student’s t-test.FA activity was significantly higher at 14 and 21 days in cultures of osteoblasts extracted from offspring exposed to maternal hyperthyroidism,while MTT conversion was significantly lower at 21 days in this group.Osteoblast cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and Op.Maternal hyperthyroidism increases the activity of matrix synthesis,alkaline phosphatase activity,and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts,extracted from the calvaria of the offspring,which may be one of the mechanisms of premature fusion of cranial sutures.

关 键 词：CRANIOSYNOSTOSIS Intramembranous growth OSSIFICATION Rat Thyroid dysfunction 

分 类 号：R68[医药卫生—骨科学]