不同因素对黄芩苷镁治疗非酒精性脂肪性肝炎大鼠模型影响研究  

Study on the effects of different factors on the treatment of non-alcoholic steatohepatitis rat model with baicalin magnesium

作　　者：管秀璐 沈诗嫄 张琳[1] 宋靖宇 刘金霞[2] 常金花[1] 毛晓霞[1] 刘翠哲[1] GUAN Xiulu;SHEN Shiyuan;ZHANG Lin;SONG Jingyu;LIU Jinxia;CHANG Jinhua;MAO Xiaoxia;LIU Cuizhe(Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development;Department of Pathogen Biology,Chengde Medical College,Chengde,Hebei 067000,China)

机构地区：[1]承德医学院河北省中药研究与开发重点实验室,河北承德067000 [2]承德医学院病原生物学教研室,河北承德067000

出　　处：《现代医药卫生》2023年第3期361-367,共7页Journal of Modern Medicine & Health

基　　金：河北省自然科学基金中医药联合基金资助项目(H2022406043);河北省中央引导地方科技发展项目-科技创新基地项目(216Z2502G);河北省高校重点学科建设项目(冀教高[2013]4);承德医学院高层次人才科研启动基金项目(202210)。

摘　　要：目的探讨在不同造模时间、给药时间和剂量下黄芩苷镁对非酒精性脂肪性肝炎(NASH)大鼠模型的治疗效果。方法采用高脂饮食诱导法建立NASH大鼠模型,将Sprague-Dawley(SD)大鼠随机分为6组,分别为空白组(16只)、模型组(16只)、黄芩苷镁1~4组(剂量分别为25、50、100、150 mg/kg,各8只)。尾静脉注射给药,观察造模时间、给药时间和剂量对大鼠的体重相关指标(体重、肝湿重、肝指数)、血清生化指标及病理结果的影响。结果造模6周时,模型组大鼠体重相关指标均较空白组增加,差异均有统计学意义(P<0.05),2组大鼠血清生化各指标均无显著变化,差异均无统计学意义(P>0.05);造模8周时,与空白组比较,模型组大鼠体重、肝湿重、肝指数、血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LCL-C)水平及非酒精性脂肪性肝病活动度积分(NAS)显著增加,高密度脂蛋白(HDL-C)水平显著降低,差异均有统计学意义(P<0.05)。给药1周时,黄芩苷镁1~4组体重相关指标均无显著变化,差异均无统计学意义(P>0.05),与模型组比较,黄芩苷镁3组大鼠血清ALT、LDL-C水平下降,差异有统计学意义(P<0.05),AST、TC、TG水平下降,HDL-C水平升高,但差异均无统计学意义(P>0.05);黄芩苷镁4组大鼠血清TG和LDL-C水平降低,HDL-C水平升高,差异有统计学意义(P<0.05)。给药2周时,与模型组比较,黄芩苷镁2组大鼠肝湿重及ALT、AST、TC、TG水平及NAS均显著降低,差异均有统计学意义(P<0.05),黄芩苷镁3组、黄芩苷镁4组大鼠体重、肝湿重、肝指数,以及ALT、AST、TC、TG、LDL-C水平及NAS明显降低,HDL-C水平明显升高,差异均有统计学意义(P<0.05)。结论造模8周比6周更接近于NASH模型,150 mg/kg的黄芩苷镁给药2周对NASH大鼠的治疗效果较好,且其作用具有剂量依赖性。Objective To explore the therapeutic effects of baicalin magnesium on non-alcoholic steatohepatitis(NASH) rats at different modeling time, administration time and doses.Methods NASH rat models were established by the method of high-fat diet induction.Sprague-Dawley(SD) rats were randomly divided into six groups: the blank group(16 rats),the model group(16 rats) and the baicalin magnesium 1-4 groups(eight rats in each group at the doses of 25,50,100 and 150 mg/kg, respectively).The medicine was injected into caudal vein of the rats to investigate the effects of modeling time, administration time and doses on body weight related indexes(body weight, wet weight of liver, liver index),serum biochemical indexes and pathological results.Results At six weeks, compared with the blank group, the weight related indexes of rats in the model group were increased, and the difference was statistically significant(P<0.05).There were no significant changes in serum biochemical indexes of rats between two groups(P>0.05).At eight weeks, compared with the blank group, the liver index, aspartate aminotransferase(AST),alanine aminotransferase(ALT),total cholesterol(TC),triglyceride(TG),low density lipoprotein(LCL-C) in serum and non-alcoholic fatty liver disease activity score(NAS) of the model group were significantly increased, while high density lipoprotein(HDL-C) was significantly decreased, and the differences were statistically significant(P<0.05).After one week of administration, there were no significant differences in the weight related indexes of baicalin magnesium 1-4 groups(P>0.05).Compared with the model group, the levels of ALT and LDL-C in serum of rats in baicalin magnesium 3 group decreased, and the differences were statistically significant(P<0.05),the levels of AST,TC,TG decreased, HDL-C levels increased, but the difference was not statistically significant(P>0.05).The levels of ALT,AST,TC,TG and LDL-C in serum of rats in baicalin magnesium 4 group decreased, while the levels of HDL-C increased, with statistical si

关 键 词：黄芩苷镁 非酒精性脂肪性肝炎 高脂饮食 剂量 造模时间 

分 类 号：R285.5[医药卫生—中药学]